Sheep Elastin Microfibril Interface Located Protein 1 ELISA Kit | Emilin1 elisa kit

Sheep Elastin Microfibril Interface Located Protein 1 ELISA Kit

Cat. Num. AAA741895

• Gene Names: EMILIN1; EMI; gp115; EMILIN
• Reactivity: Sheep
• Synonyms: Elastin Microfibril Interface Located Protein 1; Sheep Elastin Microfibril Interface Located Protein 1 ELISA Kit; Emilin1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This Emilin1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Emilin1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Emilin1 elisa kit

Principle of the Assay: Emilin1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Emilin1 antibody and an Emilin1-HRP conjugate. The assay sample and buffer are incubated together with Emilin1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Emilin1 concentration since Emilin1 from samples and Emilin1-HRP conjugate compete for the anti-Emilin1 antibody binding site. Since the number of sites is limited, as more sites are occupied by Emilin1 from the sample, fewer sites are left to bind Emilin1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Emilin1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Emilin1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 5901944
• NCBI GeneID: 11117
• NCBI Accession #: NP_008977.1
• NCBI GenBank Nucleotide #: NM_007046.3
• Molecular Weight: 35,928 Da
• NCBI Official Full Name: EMILIN-1
• NCBI Official Synonym Full Names: elastin microfibril interfacer 1
• NCBI Official Symbol: EMILIN1
• NCBI Official Synonym Symbols: EMI; gp115; EMILIN
• NCBI Protein Information: EMILIN-1; elastin microfibril interface located protein; elastin microfibril interface-located protein 1
• UniProt Protein Name: EMILIN-1
• Protein Family: EMILIN
• UniProt Gene Name: EMILIN1
• UniProt Synonym Gene Names: EMI; Elastin microfibril interfacer 1
• UniProt Entry Name: EMIL1_HUMAN

NCBI Description

This gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interface domain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encoded protein associates with elastic fibers at the interface between elastin and microfibrils and may play a role in the development of elastic tissues including large blood vessels, dermis, heart and lung. [provided by RefSeq, Sep 2009]

Uniprot Description

EMILIN1: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 2p23.3-p23.2

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; extracellular region

Molecular Function: identical protein binding; protein binding; extracellular matrix constituent conferring elasticity

Biological Process: extracellular matrix organization and biogenesis; cell adhesion