Human EMILIN-1 ELISA Kit | EMILIN1 elisa kit

Human EMILIN-1 ELISA Kit

Cat. Num. AAA2883477

• Gene Names: EMILIN1; EMI; gp115; EMILIN
• Reactivity: Human
• Synonyms: EMILIN-1; Human EMILIN-1 ELISA Kit; Elastin microfibril interface-located protein 1; Elastin microfibril interfacer 1; EMILIN1; EMI; EMILIN1 elisa kit

• Reactivity: Human
• Sequence Length: 1016

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EMILIN1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 5901944
• NCBI GeneID: 11117
• NCBI Accession #: NP_008977.1
• NCBI GenBank Nucleotide #: NM_007046.3
• UniProt Accession #: Q9Y6C2; Q53SY9; Q96G58; Q96IH6; Q9UG76; A5PL03; H0Y7A0
• Molecular Weight: 35,928 Da
• NCBI Official Full Name: EMILIN-1
• NCBI Official Synonym Full Names: elastin microfibril interfacer 1
• NCBI Official Symbol: EMILIN1
• NCBI Official Synonym Symbols: EMI; gp115; EMILIN
• NCBI Protein Information: EMILIN-1
• UniProt Protein Name: EMILIN-1
• Protein Family: EMILIN
• UniProt Gene Name: EMILIN1
• UniProt Synonym Gene Names: EMI; Elastin microfibril interfacer 1
• UniProt Entry Name: EMIL1_HUMAN

NCBI Description

This gene encodes an extracellular matrix glycoprotein that is characterized by an N-terminal microfibril interface domain, a coiled-coiled alpha-helical domain, a collagenous domain and a C-terminal globular C1q domain. The encoded protein associates with elastic fibers at the interface between elastin and microfibrils and may play a role in the development of elastic tissues including large blood vessels, dermis, heart and lung. [provided by RefSeq, Sep 2009]

Uniprot Description

EMILIN1: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity.

Protein type: Motility/polarity/chemotaxis; Extracellular matrix; Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 2p23.3-p23.2

Cellular Component: collagen; extracellular matrix; extracellular region; proteinaceous extracellular matrix

Molecular Function: protein binding

Biological Process: cell adhesion; extracellular matrix organization and biogenesis