Testing Data
(The purified sheep anti-BrdU was assayed by competitive ELISA to demonstrate both specificity and sensitivity to BromodeoxyUridine. Purified antibody was incubated in solution with each of the following nucleotide inhibitors: Adenosine, Bromouridine, Bromodeoxyuridine, Thymidine, Deoxycytadine, and Uridine prior to incubation on plate coated with BrdU. The data below shows that antibody incubated with BrdU bound to the molecule in solution, and therefore shows lower signal at lower dilutions. As the sample is diluted, more of the antibody is available to bind the BrdU on the plate. The result is an increase in signal as titer increases.)
Sheep Bromodeoxyuridine-5 (BrdU) Polyclonal Antibody | anti-BrdU antibody
Sheep Polyclonal Antibody to Bromodeoxyuridine-5 (BrdU)
Applications
Western Blot
Purity
Protein G Purification followed by polishing chromatography against resin coupled to HGG
Synonyms
Bromodeoxyuridine-5 (BrdU); Polyclonal Antibody; Sheep Polyclonal Antibody to Bromodeoxyuridine-5 (BrdU); anti-BrdU antibody
Host
Sheep
Clonality
Polyclonal
Purity/Purification
Protein G Purification followed by polishing chromatography against resin coupled to HGG
Applicable Applications for anti-BrdU antibody
Western Blot (WB)
Application Notes
Western Blotting Relative MW 307 kDa
Immunogen
Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)
Preparation and Storage
4 C, or aliquot into working volumes and store at -20 C
Testing Data
(The purified sheep anti-BrdU was assayed by competitive ELISA to demonstrate both specificity and sensitivity to BromodeoxyUridine. Purified antibody was incubated in solution with each of the following nucleotide inhibitors: Adenosine, Bromouridine, Bromodeoxyuridine, Thymidine, Deoxycytadine, and Uridine prior to incubation on plate coated with BrdU. The data below shows that antibody incubated with BrdU bound to the molecule in solution, and therefore shows lower signal at lower dilutions. As the sample is diluted, more of the antibody is available to bind the BrdU on the plate. The result is an increase in signal as titer increases.)
Testing Data
(The purified sheep anti-BrdU was assayed by competitive ELISA to demonstrate both specificity and sensitivity to BromodeoxyUridine. Purified antibody was incubated in solution with each of the following nucleotide inhibitors: Adenosine, Bromouridine, Bromodeoxyuridine, Thymidine, Deoxycytadine, and Uridine prior to incubation on plate coated with BrdU. The data below shows that antibody incubated with BrdU bound to the molecule in solution, and therefore shows lower signal at lower dilutions. As the sample is diluted, more of the antibody is available to bind the BrdU on the plate. The result is an increase in signal as titer increases.)
References
1) Dolbeare, Frank (1995) Bromodeoxyuridine: A diagnostic tool in biology and medicine, Part 1: Historical perspectives, histochemical methods and cell kinetics. Histochemical Journal 27, 339-369 (1995) 2) Harms, G. Van Goor, H. Koudstaal, J. de Ley, L. and Hardonk, M.J. (1986) Immunohistochemical demonstration of DNA-incorporated 5-bromodeoxyuridine in frozen and plastic embedded sections. Histochemistry (1986) 85, 139-143 3) Matsuura, Sachiko. Suzuki, Kazuo (1997) Immunohistochemical Analysis of DNA Synthesis during Chronic Stimulation with Isoproterenol in Mouse Submandibular Gland. Journal of Histochemistry & Cytochemistry Volume 45(8): 1137-1145, 1997
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Product Notes
The BrdU (Catalog #AAA410071) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bromodeoxyuridine-5 (BrdU) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blotting Relative MW 307 kDa. Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bromodeoxyuridine-5 (BrdU), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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