Human E3 ubiquitin-protein ligase UBR4 ELISA Kit | UBR4 elisa kit

Human E3 ubiquitin-protein ligase UBR4 ELISA Kit

Cat. Num. AAA762578

• Gene Names: UBR4; p600; ZUBR1; RBAF600
• Reactivity: Human
• Synonyms: E3 ubiquitin-protein ligase UBR4; Human E3 ubiquitin-protein ligase UBR4 ELISA Kit; UBR4 (E3 ubiquitin-protein ligase UBR4)/RBAF600/p600/N-recognin-4/Zinc finger UBR1-type protein 1/Retinoblastoma-associated factor of 600 kDa/600 kDa retinoblastoma protein-associated factor; UBR4 elisa kit

• Reactivity: Human
• Sequence Length: 5183

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for UBR4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-UBR4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-UBR4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the UBR4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of UBR4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 82659109
• NCBI GeneID: 23352
• NCBI Accession #: NP_065816.2
• NCBI GenBank Nucleotide #: NM_020765.2
• Molecular Weight: 23,957 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR4
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 4
• NCBI Official Symbol: UBR4
• NCBI Official Synonym Symbols: p600; ZUBR1; RBAF600
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR4
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR4
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: UBR4
• UniProt Synonym Gene Names: KIAA0462; KIAA1307; RBAF600; ZUBR1; RBAF600; p600
• UniProt Entry Name: UBR4_HUMAN

NCBI Description

The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]

Uniprot Description

UBR4: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Belongs to the UBR4 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; Ligase; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: centrosome; cytoplasm; integral to membrane; membrane; nucleoplasm

Molecular Function: calmodulin binding; ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; viral reproduction