Testing Data
Rat anti-Mouse BP-1 (Ly-51, 6C3) Monoclonal Antibody
Rat Anti-Mouse BP-1/Ly-51-PE
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
BP-1 (Ly-51; 6C3); Monoclonal Antibody; Rat Anti-Mouse BP-1/Ly-51-PE; Rat Anti-Mouse BP-1/Ly-51 - Phycoerythrin-Labeled; Rat Anti-Mouse BP-1 (Ly-51; anti-BP-1 (Ly-51; 6C3) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1 kappa
Clone Number
FG35.4
Specificity
BP-1/Ly-51/6C3 antigen, (aminopeptidase A, E.C.3.4.11.7, glutamylaminopeptidase)
BP-1, also known as Ly-51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kDa.1, 2 Originally identified on the surface of pre-B cell lymphomas1, 3 the cDNA encoding BP-1/Ly-51/6C3 has been cloned and found to represent aminopeptidase A.4, 5 It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity.6-8 Subsets of normal bone marrow pre-B and B cells express low levels of BP-1/Ly-51/6C3, which is rapidly upregulated on pre-B cells in the presence of IL- 7.9, 10 The BP-1/6C3 marker is commonly used to identify B cells in different stages of development.11
BP-1, also known as Ly-51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kDa.1, 2 Originally identified on the surface of pre-B cell lymphomas1, 3 the cDNA encoding BP-1/Ly-51/6C3 has been cloned and found to represent aminopeptidase A.4, 5 It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity.6-8 Subsets of normal bone marrow pre-B and B cells express low levels of BP-1/Ly-51/6C3, which is rapidly upregulated on pre-B cells in the presence of IL- 7.9, 10 The BP-1/6C3 marker is commonly used to identify B cells in different stages of development.11
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for anti-BP-1 (Ly-51, 6C3) antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Testing Data
Testing Data
References
1. Cooper, M. D., D. Mulvaney, A. Coutinho, and P. A. Cazenave. 1986. Nature 321:616.
2. Wu, Q., G. F. Tidmarsh, P. A. Welch, J. H. Pierce, I. L. Weissman, and M. D. Cooper. 1989. J. Immunol. 143:3303.
3. Pillemer, E., C. Whitelock, and I. L. Weissman. 1984. Proc. Natl. Acad. Sci. USA. 81:4434.
4. Wu, Q., J. M. Lahti, G. M. Air, P. D. Burrows, and M. D. Cooper. 1990. Proc. Natl. Acad. Sci. USA. 87:993.
5. Wu, Q., L. Li, M. D. Cooper, M. Pierres, and J. P. Gorvel. 1991. Proc. Natl. Acad. Sci. U. S. A. 88:676.
6. Whitlock, C. A., G. F. Tidmarsh, C. Muller-Sieburg, and I. L. Weissman. 1987. Cell 48:1009.
7. Adkins, B., G. F. Tidmarsh, and I. L. Weissman. 1988. Immunogenetics 27:180.
8. Li, L., Q. Wu, J. Wang, R. P. Bucy, and M. D. Cooper. 1993. Tissue Antigens. 42:488.
9. Sherwood, P. J. and I. L. Weissman. 1990. Int. Immunol. 2:399.
10. Welch, P. A., P. D. Burrows, A. Namen, S. Gillis, and M. D. Cooper. 1996. Int. Immunol. 2:697.
11. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med. 173:1213.
12. Shu, F. and J. F. Kearney. 1996. Personal communication.
2. Wu, Q., G. F. Tidmarsh, P. A. Welch, J. H. Pierce, I. L. Weissman, and M. D. Cooper. 1989. J. Immunol. 143:3303.
3. Pillemer, E., C. Whitelock, and I. L. Weissman. 1984. Proc. Natl. Acad. Sci. USA. 81:4434.
4. Wu, Q., J. M. Lahti, G. M. Air, P. D. Burrows, and M. D. Cooper. 1990. Proc. Natl. Acad. Sci. USA. 87:993.
5. Wu, Q., L. Li, M. D. Cooper, M. Pierres, and J. P. Gorvel. 1991. Proc. Natl. Acad. Sci. U. S. A. 88:676.
6. Whitlock, C. A., G. F. Tidmarsh, C. Muller-Sieburg, and I. L. Weissman. 1987. Cell 48:1009.
7. Adkins, B., G. F. Tidmarsh, and I. L. Weissman. 1988. Immunogenetics 27:180.
8. Li, L., Q. Wu, J. Wang, R. P. Bucy, and M. D. Cooper. 1993. Tissue Antigens. 42:488.
9. Sherwood, P. J. and I. L. Weissman. 1990. Int. Immunol. 2:399.
10. Welch, P. A., P. D. Burrows, A. Namen, S. Gillis, and M. D. Cooper. 1996. Int. Immunol. 2:697.
11. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med. 173:1213.
12. Shu, F. and J. F. Kearney. 1996. Personal communication.
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Product Notes
The BP-1 (Ly-51, 6C3) (Catalog #AAA673493) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse BP-1/Ly-51-PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BP-1 (Ly-51, 6C3) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the BP-1 (Ly-51, 6C3) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BP-1 (Ly-51, 6C3), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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