Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat UBR4/ZUBR1 Polyclonal Antibody | anti-UBR4 antibody
Goat anti-UBR4/ZUBR1 Antibody
Immunohistochemistry: In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10ug/ml.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product.
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]
Uniprot Description
UBR4: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Regulates integrin-mediated signaling. May play a role in activation of FAK in response to cell-matrix interactions. Belongs to the UBR4 family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ligase; Membrane protein, integral; Ubiquitin conjugating system; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p36.13
Cellular Component: centrosome; cytoplasm; integral to membrane; membrane; nucleoplasm
Molecular Function: calmodulin binding; ligase activity; protein binding; ubiquitin-protein ligase activity; zinc ion binding
Biological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process; viral reproduction
Research Articles on UBR4
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Product Notes
The UBR4 ubr4 (Catalog #AAA421940) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-UBR4/ZUBR1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's UBR4/ZUBR1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10ug/ml. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product. Researchers should empirically determine the suitability of the UBR4 ubr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NTTRATSREE KNLQ. It is sometimes possible for the material contained within the vial of "UBR4/ZUBR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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