Rabbit anti-Mouse Aspartylglucosaminidase Polyclonal Antibody | anti-AGA antibody

Aspartylglucosaminidase (AGA) Polyclonal Antibody

Cat. Num. AAA2032884

• Gene Names: Aga; AW060726
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Aspartylglucosaminidase; Polyclonal Antibody; Aspartylglucosaminidase (AGA) Polyclonal Antibody; AGU; ASRG; GA; N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase; anti-AGA antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against AGA. It has been selected for its ability to recognize AGA in immunohistochemical staining and western blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence Length: 64

• Applicable Applications for anti-AGA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western Blot: 1:50-400; Immunohistochemistry in formalin fixed frozen section: 1:50-500; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunoglobulin Type: IgG
• Conjugate: No Conjugate
• Cross Reactivity: Mouse
• Organism Species: Mouse
• Fragment: AGA (Ser24~Gly260)
• Quality Control: Content: The quality control contains recombinant AGA (Ser24~Gly260) disposed in loading buffer.; Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.; 5uL per well when used in enhanced chemilumescent (ECL).; Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.; Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2064891
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Kidney Tissue; Lane2: Mouse Placenta Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant AGA, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Heart Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 54292135
• NCBI GeneID: 11593
• NCBI Accession #: NP_001005847.1
• NCBI GenBank Nucleotide #: NM_001005847.2
• UniProt Accession #: Q64191
• Molecular Weight: 37,022 Da
• NCBI Official Full Name: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1 preproprotein
• NCBI Official Synonym Full Names: aspartylglucosaminidase
• NCBI Official Symbol: Aga
• NCBI Official Synonym Symbols: AW060726
• NCBI Protein Information: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
• UniProt Protein Name: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase
• Protein Family: N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
• UniProt Gene Name: Aga
• UniProt Synonym Gene Names: AGA

NCBI Description

This gene encodes an amidase enzyme that participates in the breakdown of glycoproteins in the cell. The encoded protein undergoes proteolytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit accumulation of aspartylglucosamine along with lysosomal vacuolization, axonal swelling in the gracile nucleus and impaired neuromotor coordination. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

Uniprot Description

AGA: a catabolic enzyme, aspartylglucosaminidase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. The lysosomal storage disease aspartylglycosaminuria is caused by a deficiency in the AGA enzyme.

Protein type: EC 3.5.1.26; Glycan Metabolism - other glycan degradation; Hydrolase

Chromosomal Location of Human Ortholog: 8|8 B1.3

Cellular Component: endoplasmic reticulum; extracellular exosome; extracellular space; lysosome

Molecular Function: hydrolase activity; N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity; peptidase activity; protein self-association

Biological Process: protein deglycosylation; protein maturation; proteolysis