Human DNA Methyltransferase 3A ELISA Kit | Dnmt3a elisa kit

Human DNA Methyltransferase 3A ELISA Kit

Cat. Num. AAA3802203

• Gene Names: Dnmt3a; MmuIIIA
• Reactivity: Human
• Synonyms: DNA Methyltransferase 3A; Human DNA Methyltransferase 3A ELISA Kit; Dnmt3a elisa kit

• Reactivity: Human
• Sequence Length: 908

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 5 U/L-80 U/L
• Sensitivity: 0.1 U/L

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Dnmt3a elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofDNA METHYLTRANSFERASE 3Ain the sample, This DNA METHYLTRANSFERASE 3AELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusDNA METHYLTRANSFERASE 3Aconcentration. The concentration ofDNA METHYLTRANSFERASE 3Ain the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 418203928
• NCBI GeneID: 13435
• NCBI Accession #: NP_001258682.1
• NCBI GenBank Nucleotide #: NP_001258682.1
• UniProt Accession #: O88508
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3A isoform 1
• NCBI Official Synonym Full Names: DNA methyltransferase 3A
• NCBI Official Symbol: Dnmt3a
• NCBI Official Synonym Symbols: MmuIIIA
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3A
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3A
• Protein Family: DNA
• UniProt Gene Name: Dnmt3a
• UniProt Synonym Gene Names: Dnmt3a; DNA MTase MmuIIIA; M.MmuIIIA
• UniProt Entry Name: DNM3A_MOUSE

NCBI Description

This is one of two related genes encoding de novo DNA methyltransferases, which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene causes developmental defects in multiple different organ systems. There is a pseudogene for this gene located on chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Nov 2012]

Uniprot Description

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase, DNA; EC 2.1.1.37; Methyltransferase; Amino Acid Metabolism - cysteine and methionine

Cellular Component: nucleoplasm; XY body; heterochromatin; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: transferase activity; DNA (cytosine-5-)-methyltransferase activity; methyltransferase activity; identical protein binding; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding

Biological Process: methylation; genetic imprinting; maintenance of DNA methylation; transcription, DNA-dependent; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; cytosine methylation within a CG sequence; methylation-dependent chromatin silencing; regulation of transcription, DNA-dependent; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; DNA methylation during embryonic development; DNA methylation on cytosine