Rabbit anti-Human BUB1 Polyclonal Antibody | anti-BUB1 antibody

BUB1 polyclonal antibody

Cat. Num. AAA3001007

• Gene Names: BUB1; BUB1A; BUB1L; hBUB1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: BUB1; Polyclonal Antibody; BUB1 polyclonal antibody; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A; anti-BUB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: BUB1 Polyclonal Antibody detects endogenous levels of BUB1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-BUB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; ELISA: 1/40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human BUB1. AA range: 781-830
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of the lysates from HUVECcells using BUB1 antibody.)

Related Product Information for anti-BUB1 antibody

Background: BUB1 mitotic checkpoint serine/threonine kinase(BUB1) Homo sapiens. This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

NCBI and Uniprot Product Information

• NCBI GI #: 4757878
• NCBI GeneID: 699
• NCBI Accession #: NP_004327.1
• NCBI GenBank Nucleotide #: NM_004336.3
• UniProt Accession #: O43683; O43430; O43643; O60626; Q53QE4
• Molecular Weight: 122,375 Da
• NCBI Official Full Name: mitotic checkpoint serine/threonine-protein kinase BUB1
• NCBI Official Synonym Full Names: BUB1 mitotic checkpoint serine/threonine kinase
• NCBI Official Symbol: BUB1
• NCBI Official Synonym Symbols: BUB1A; BUB1L; hBUB1
• NCBI Protein Information: mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog
• UniProt Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1
• Protein Family: Checkpoint serine/threonine-protein kinase
• UniProt Gene Name: BUB1
• UniProt Synonym Gene Names: BUB1L; hBUB1
• UniProt Entry Name: BUB1_HUMAN

NCBI Description

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Cell cycle regulation; Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; mitosis; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of chromosome segregation; regulation of sister chromatid cohesion; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation