Human Dipeptidase 1, Renal (DPEP1) ELISA Kit | DPEP1 elisa kit

Human Dipeptidase 1, Renal (DPEP1) ELISA Kit

Cat. Num. AAA456057

• Gene Names: DPEP1; MDP; RDP; MBD1
• Reactivity: Human
• Synonyms: Dipeptidase 1; Renal (DPEP1); Human Dipeptidase 1; Renal (DPEP1) ELISA Kit; DPEP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DPEP1. No significant cross-reactivity or interference between DPEP1 and analogues was observed.
• Sequence Length: 411

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.050ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DPEP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DPEP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPEP1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DPEP1 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPEP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DPEP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DPEP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DPEP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 189458885
• NCBI GeneID: 1800
• NCBI Accession #: NP_001121613.1
• NCBI GenBank Nucleotide #: NM_001128141.2
• UniProt Accession #: P16444; Q96AK2; D3DX80
• Molecular Weight: 45,674 Da
• NCBI Official Full Name: dipeptidase 1
• NCBI Official Synonym Full Names: dipeptidase 1 (renal)
• NCBI Official Symbol: DPEP1
• NCBI Official Synonym Symbols: MDP; RDP; MBD1
• NCBI Protein Information: dipeptidase 1
• UniProt Protein Name: Dipeptidase 1
• Protein Family: Dipeptidase
• UniProt Gene Name: DPEP1
• UniProt Synonym Gene Names: MDP; RDP; hRDP

NCBI Description

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.