Human Dipeptidase 1 ELISA Kit | DPEP1 elisa kit

Human Dipeptidase 1 ELISA Kit

Cat. Num. AAA765214

• Gene Names: DPEP1; MDP; RDP; MBD1
• Reactivity: Human
• Synonyms: Dipeptidase 1; Human Dipeptidase 1 ELISA Kit; DPEP1/Renal dipeptidase/hRDP/Dehydropeptidase-I/Microsomal dipeptidase/MDP/RDP; DPEP1 elisa kit

• Reactivity: Human
• Sequence Length: 411

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DPEP1 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPEP1. During the reaction,Human DPEP1 in the sample or standard competes with a fixed amount of biotin-labeled Human DPEP1 for sites on a pre-coated Monoclonal antibody specific to Human DPEP1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human DPEP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 189458885
• NCBI GeneID: 1800
• NCBI Accession #: NP_001121613.1
• NCBI GenBank Nucleotide #: NM_001128141.2
• Molecular Weight: 45,674 Da
• NCBI Official Full Name: dipeptidase 1
• NCBI Official Synonym Full Names: dipeptidase 1 (renal)
• NCBI Official Symbol: DPEP1
• NCBI Official Synonym Symbols: MDP; RDP; MBD1
• NCBI Protein Information: dipeptidase 1
• UniProt Protein Name: Dipeptidase 1
• Protein Family: Dipeptidase
• UniProt Gene Name: DPEP1
• UniProt Synonym Gene Names: MDP; RDP; hRDP
• UniProt Entry Name: DPEP1_HUMAN

NCBI Description

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DPEP1: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. Belongs to the peptidase M19 family.

Protein type: EC 3.4.13.19; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: apical part of cell; apical plasma membrane; extracellular space; microvillus membrane; plasma membrane

Molecular Function: caspase inhibitor activity; dipeptidyl-peptidase activity; metalloexopeptidase activity; protein binding; zinc ion binding

Biological Process: antibiotic metabolic process; arachidonic acid metabolic process; glutathione metabolic process; homocysteine metabolic process; leukotriene metabolic process; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell migration; proteolysis; xenobiotic metabolic process