Human Dipeptidase 1, Renal (DPEP1) ELISA Kit | DPEP1 elisa kit

Human Dipeptidase 1, Renal (DPEP1) ELISA Kit

Cat. Num. AAA2000022

• Gene Names: DPEP1; MDP; RDP; MBD1
• Reactivity: Human
• Synonyms: Dipeptidase 1; Renal (DPEP1); Human Dipeptidase 1; Renal (DPEP1) ELISA Kit; RDP; MBD1; MDP; Dehydropeptidase-I; Microsomal dipeptidase; Renal dipeptidase; DPEP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dipeptidase 1, Renal (DPEP1).; No significant cross-reactivity or interference between Dipeptidase 1, Renal (DPEP1) and analogues was observed.
• Sequence Length: 411

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dipeptidase 1, Renal (DPEP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dipeptidase 1, Renal (DPEP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPEP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidase 1, Renal (DPEP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidase 1, Renal (DPEP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidase 1, Renal (DPEP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidase 1, Renal (DPEP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DPEP1 elisa kit

Enzyme & Kinase; Metabolic pathway; Infection immunity; Kidney biomarker

NCBI and Uniprot Product Information

• NCBI GI #: 4758190
• NCBI GeneID: 1800
• NCBI Accession #: NP_004404.1
• NCBI GenBank Nucleotide #: NM_004413.3
• Molecular Weight: 45,674 Da
• NCBI Official Full Name: dipeptidase 1
• NCBI Official Synonym Full Names: dipeptidase 1 (renal)
• NCBI Official Symbol: DPEP1
• NCBI Official Synonym Symbols: MDP; RDP; MBD1
• NCBI Protein Information: dipeptidase 1
• UniProt Protein Name: Dipeptidase 1
• Protein Family: Dipeptidase
• UniProt Gene Name: DPEP1
• UniProt Synonym Gene Names: MDP; RDP; hRDP
• UniProt Entry Name: DPEP1_HUMAN

NCBI Description

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DPEP1: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. Belongs to the peptidase M19 family.

Protein type: Membrane protein, GPI anchor; EC 3.4.13.19; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; metalloexopeptidase activity; zinc ion binding; dipeptidyl-peptidase activity; caspase inhibitor activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process; arachidonic acid metabolic process; leukotriene metabolic process; negative regulation of caspase activity; homocysteine metabolic process; proteolysis; negative regulation of cell migration; antibiotic metabolic process; negative regulation of apoptosis