DPEP1 recombinant protein

DPEP1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121457

• Gene Names: DPEP1; MDP; RDP; MBD1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DPEP1; DPEP1 Protein; Human; Recombinant (His Tag); Human Dehydropeptidase-I/DPEP1 Protein (His Tag); MBD1 Protein; MDP Protein; RDP Protein; dipeptidase 1; DPEP1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser385

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human DPEP1 (P16444)(Met1-Ser385) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for DPEP1 recombinant protein

Background: Dehydropeptidase-I, also known as DPEP1, is a kidney membrane enzyme. Its expression in normal colonic mucosa is very low, but it is highly expressed in colorectal adenoma and cancer specimens and is negatively correlated with parameters of pathological aggressiveness and poor prognosis. The overexpression of DPEP1 suppressed tumor cells invasiveness and increased sensitivity to chemotherapeutic agent Gemcitabine. Growth factor EGF treatment decreased DPEP1 expression. Dehydropeptidase-I may be a candidate target in PDAC for designing improved treatments. It uses zinc as a cofactor and acts as a disulfide-linked homodimer.

References

Toiyama Y. et al., 2011, J Gastroenterol. 46 (2): 153-63. Zhang G. et al., 2012, PLoS One. 7 (2): e31507. Okamoto T. et al., 2011, Mod Pathol. 24 (2): 267-76.

NCBI and Uniprot Product Information

• NCBI GI #: 4758190
• NCBI GeneID: 1800
• NCBI Accession #: NP_004404.1
• NCBI GenBank Nucleotide #: NM_004413.3
• UniProt Accession #: P16444; Q96AK2; D3DX80
• Molecular Weight: 45,674 Da
• NCBI Official Full Name: dipeptidase 1
• NCBI Official Synonym Full Names: dipeptidase 1 (renal)
• NCBI Official Symbol: DPEP1
• NCBI Official Synonym Symbols: MDP; RDP; MBD1
• NCBI Protein Information: dipeptidase 1; hRDP; renal dipeptidase; dehydropeptidase-I; microsomal dipeptidase
• UniProt Protein Name: Dipeptidase 1
• Protein Family: Dipeptidase
• UniProt Gene Name: DPEP1
• UniProt Synonym Gene Names: MDP; RDP; hRDP
• UniProt Entry Name: DPEP1_HUMAN

NCBI Description

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

DPEP1: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. Belongs to the peptidase M19 family.

Protein type: Protease; EC 3.4.13.19; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space; microvillus membrane; apical part of cell; apical plasma membrane; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; metalloexopeptidase activity; zinc ion binding; dipeptidyl-peptidase activity; caspase inhibitor activity

Biological Process: glutathione metabolic process; xenobiotic metabolic process; arachidonic acid metabolic process; leukotriene metabolic process; homocysteine metabolic process; negative regulation of caspase activity; proteolysis; negative regulation of cell migration; antibiotic metabolic process; negative regulation of apoptosis