Mouse anti-Human CD45 Monoclonal Antibody | anti-CD45 antibody

anti-human CD45 RA-R-PE

Cat. Num. AAA666582

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD45; Monoclonal Antibody; anti-human CD45 RA-R-PE; Monoclonal anti-human CD45RA/R-PE; anti-CD45 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 158.4D3
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2ml (varies by lot)
• Sequence Length: 72

• Applicable Applications for anti-CD45 antibody: Flow Cytometry (FC/FACS), PS
• Application Notes: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
• Performance: Five x 105 cultured human Raji cells per tube were incubated 45 minutes on ice with 80 ul of anti-CD45RA/R-PE at a dilution factor of 1:50 (5ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.61 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD45RA antibody.
• Conjugation: PE
• Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 250 ug/ml with an A565/A280 ratio of 3.28.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Stimulated human leukocytes
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD45 antibody

Information: Human CD45RA is expressed on all cells of hematopoietic origin, except erythrocytes. CD45RA is a transmembrane tyrosine phosphate which can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. The CD45RA isoform predominates on naive/resting T cells and medullary thymocytes. Antibody 158.4D3 recognizes all CD45 molecules containing the A region exon (1,2).

References

1) Leukocyte Typing III (A.J. McMichael, et al, eds.) Oxford University Press, Oxford, (1987) p. 788-817. 2) A. Gaya, et al, (1990) Intl Immunol 2:685-689. 3.) I.S. Trowbridge & M.L. Thomas, (1994) Annu Rev Immunol 12: 85-116.

NCBI and Uniprot Product Information

• NCBI GI #: 44242008
• NCBI GeneID: 5788
• Molecular Weight: 130,898 Da
• NCBI Official Full Name: CD45, partial
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Receptor protein phosphatase, tyrosine; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; positive regulation of T cell proliferation; negative regulation of cytokine and chemokine mediated signaling pathway; hemopoietic progenitor cell differentiation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive