Human Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) ELISA Kit | CRELD1 elisa kit

Human Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) ELISA Kit

Cat. Num. AAA2021614

• Gene Names: CRELD1; AVSD2; CIRRIN
• Reactivity: Human
• Synonyms: Cysteine Rich With EGF Like Domains Protein 1 (CRELD1); Human Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) ELISA Kit; AVSD2; CIRRIN; Atrioventricular Septal Defect 2; CRELD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich With EGF Like Domains Protein 1 (CRELD1).; No significant cross-reactivity or interference between Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) and analogues was observed.
• Sequence Length: 420

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRELD1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich With EGF Like Domains Protein 1 (CRELD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich With EGF Like Domains Protein 1 (CRELD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich With EGF Like Domains Protein 1 (CRELD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich With EGF Like Domains Protein 1 (CRELD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CRELD1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 116734855
• NCBI GeneID: 78987
• NCBI Accession #: NP_001070883.1
• NCBI GenBank Nucleotide #: NM_001077415.2
• Molecular Weight: 45,942 Da
• NCBI Official Full Name: cysteine-rich with EGF-like domain protein 1 isoform 2
• NCBI Official Synonym Full Names: cysteine-rich with EGF-like domains 1
• NCBI Official Symbol: CRELD1
• NCBI Official Synonym Symbols: AVSD2; CIRRIN
• NCBI Protein Information: cysteine-rich with EGF-like domain protein 1
• UniProt Protein Name: Cysteine-rich with EGF-like domain protein 1
• Protein Family: Cysteine-rich with EGF-like domain protein
• UniProt Gene Name: CRELD1
• UniProt Synonym Gene Names: CIRRIN
• UniProt Entry Name: CREL1_HUMAN

NCBI Description

This gene encodes a member of a subfamily of epidermal growth factor-related proteins. The encoded protein is characterized by a cysteine-rich with epidermal growth factor-like domain. This protein may function as a cell adhesion molecule. Mutations in this gene are the cause of atrioventricular septal defect. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

Uniprot Description

CRELD1: Defects in CRELD1 may be the cause of susceptibility to atrioventricular septal defect type 2 (AVSD2). AVSD is a spectrum of cardiac malformations that result in a persistent common atrioventricular canal. The complete form of AVSD involves underdevelopment of the lower part of the atrial septum and the upper part of the ventricular septum. A less severe form, known as partial AVSD or ostium primum atrial septal defect has a deficiency of the atrial septum. Complete AVSD are clinically apparent at birth, whereas less severe forms, such as an isolated cleft mitral valve or small defects of the atrial or ventricular septa may go undetected. Belongs to the CRELD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: integral to membrane

Molecular Function: calcium ion binding

Disease: Atrioventricular Septal Defect, Susceptibility To, 2