Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lipoprotein alpha (Lp-a) Standard Curve.)
Human Lipoprotein alpha (Lp-a) ELISA Kit | Lp-a elisa kit
Human Lipoprotein alpha (Lp-a) ELISA Kit
Reactivity
Human
Synonyms
Lipoprotein alpha (Lp-a); Human Lipoprotein alpha (Lp-a) ELISA Kit; Lp-a; LP-A; AK38; APOA; LP; OTTHUMP00000017543; antiangiogenic AK38 protein; apolipoprotein(a); lipoprotein Lp(a); Lp-a elisa kit
Reactivity
Human
Specificity
Human Lipoprotein alpha (Lp-a) ELISA Kit has high sensitivity and excellent specificity for detection of Human Lp-a. No significant cross-reactivity or interference between Human Lp-a and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lipoprotein alpha (Lp-a) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lipoprotein alpha (Lp-a) Standard Curve.)
Related Product Information for Lp-a elisa kit
Description: This Human Lipoprotein alpha (Lp-a) ELISA Kit employs a two-site sandwich ELISA to quantitate Lp-a in samples. An antibody specific for Lp-a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Lp-a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Lp-a is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Lp-a bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Lipoprotein(a) consists of an LDL-like particle and the specific apolipoprotein(a), which is covalently bound to the apoB of the LDL like particle. Lp(a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein(a) gene located on chromosome 6q26-27. Apo(a) proteins vary in size due to a size polymorphism, which is caused by a variable number of so called kringle IV repeats in the LPA gene. This size variation at the gene level is expressed on the protein level as well, resulting in apo(a) proteins with 10 to > 50 kringle IV repeats. These variable apo(a) sizes are known as "apo(a) isoforms". There is a general inverse correlation between the size of the apo(a)isoform and the Lp(a) plasma concentration which is caused by a variable rate of degradation before the apo(a) protein has matured for Lp(a) assembly.
Background: Lipoprotein(a) consists of an LDL-like particle and the specific apolipoprotein(a), which is covalently bound to the apoB of the LDL like particle. Lp(a) plasma concentrations are highly heritable and mainly controlled by the apolipoprotein(a) gene located on chromosome 6q26-27. Apo(a) proteins vary in size due to a size polymorphism, which is caused by a variable number of so called kringle IV repeats in the LPA gene. This size variation at the gene level is expressed on the protein level as well, resulting in apo(a) proteins with 10 to > 50 kringle IV repeats. These variable apo(a) sizes are known as "apo(a) isoforms". There is a general inverse correlation between the size of the apo(a)isoform and the Lp(a) plasma concentration which is caused by a variable rate of degradation before the apo(a) protein has matured for Lp(a) assembly.
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Product Notes
The Human Lp-a (Catalog #AAA9715450) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715450 ELISA Kit recognizes Human Lp-a. It is sometimes possible for the material contained within the vial of "Lipoprotein alpha (Lp-a), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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