Western Blot (WB)
(Western Blot analysis of A549 cell line lysates (35ug/lane) usingMBS643423.)
Rabbit anti-Human RHD Polyclonal Antibody | anti-RHD antibody
RHD, ID (RHD, Blood group Rh(D) polypeptide, RHXIII, Rh polypeptide 2, Rhesus D antigen, CD240D) (Azide free) (HRP)
Gene Names
RHD; RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
Reactivity
Human
Applications
Western Blot
Purity
Antibody prior to conjugation was purified by Protein A affinity chromatography followed by peptide affinity purification.
Synonyms
RHD; Polyclonal Antibody; ID (RHD; Blood group Rh(D) polypeptide; RHXIII; Rh polypeptide 2; Rhesus D antigen; CD240D) (Azide free) (HRP); CD240D; anti-RHD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human RHD.
Purity/Purification
Antibody prior to conjugation was purified by Protein A affinity chromatography followed by peptide affinity purification.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-RHD antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide derived from a portion of amino acids within the region aa173-201 amino acids in the central region of human RHD.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of A549 cell line lysates (35ug/lane) usingMBS643423.)
Western Blot (WB)
(Western Blot analysis of A549 cell line lysates (35ug/lane) usingMBS643423.)
Product Categories/Family for anti-RHD antibody
References
Flegr, J., et al. Folia Parasitol. 57(2):143-150(2010). Liu, H.C., et al. Biochim. Biophys. Acta 1800(6):565-573(2010). Schmid, P., et al. Transfusion 50(1):267-269(2010). Wang, X.D., et al. Eur. J. Clin. Invest. 39(7):607-617(2009). Wagner, F.F., et al. Blood 99(6):2272-2273(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,694 Da
NCBI Official Full Name
blood group Rh(D) polypeptide isoform 2
NCBI Official Synonym Full Names
Rh blood group D antigen
NCBI Official Symbol
RHD
NCBI Official Synonym Symbols
RH; Rh4; RH30; RhII; RhPI; DIIIc; RHCED; RHDel; RHPII; RhDCw; CD240D; RHXIII; RHDVA(TT); RhK562-II
NCBI Protein Information
blood group Rh(D) polypeptide
UniProt Protein Name
Blood group Rh(D) polypeptide
UniProt Gene Name
RHD
UniProt Synonym Gene Names
RhPII
NCBI Description
The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
Research Articles on RHD
Similar Products
Product Notes
The RHD rhd (Catalog #AAA6341866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHD, ID (RHD, Blood group Rh(D) polypeptide, RHXIII, Rh polypeptide 2, Rhesus D antigen, CD240D) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RHD rhd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
