Human Cystatin SN (CST1) ELISA Kit | CST1 elisa kit

Human Cystatin SN (CST1) ELISA Kit

Cat. Num. AAA7226643

• Reactivity: Human
• Synonyms: Cystatin SN (CST1); Human Cystatin SN (CST1) ELISA Kit; CST1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This CST1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CST1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CST1 elisa kit

Principle of the assay: CST1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CST1 antibody and an CST1-HRP conjugate. The assay sample and buffer are incubated together with CST1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CST1 concentration since CST1 from samples and CST1-HRP conjugate compete for the anti-CST1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CST1 from the sample, fewer sites are left to bind CST1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CST1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CST1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 19882251
• NCBI GeneID: 1469
• NCBI Accession #: NP_001889.2
• NCBI GenBank Nucleotide #: NM_001898.2
• Molecular Weight: 16,388 Da
• NCBI Official Full Name: cystatin-SN
• NCBI Official Synonym Full Names: cystatin SN
• NCBI Official Symbol: CST1
• NCBI Protein Information: cystatin-SN; cystatin 1; cystatin-1; cystain-SA-I; cystatin SA-I; salivary cystatin-SA-1; cysteine proteinase inhibitor, type 2 family
• UniProt Protein Name: Cystatin-SN
• Protein Family: Cystatin
• UniProt Gene Name: CST1
• UniProt Entry Name: CYTN_HUMAN

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq, Jul 2008]

Uniprot Description

CST1: Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: cysteine protease inhibitor activity

Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste