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SDS-Page
(Figure. SDS-PAGE)
Ribonuclease H Recombinant Protein | RNASEH recombinant protein
Recombinant Ribonuclease H (RNASEH)
Gene Names
RNASEH1; RNH1; H1RNA; PEOB2
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Ribonuclease H; Recombinant Ribonuclease H (RNASEH); Rnase-H; RNASEH1; RNH1; Ribonuclease H type II; RNASEH recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 100ug/mL (varies by lot)
Applicable Applications for RNASEH recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Met1~Asp286
Tags
N-terminal His and GST Tag
Subcellular Location
Cytoplasm
Traits
Freeze-dried powder
Predicted isoelectric point
9.3
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0-0.2 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-Page
(Figure. SDS-PAGE)
SDS-Page
(Figure. SDS-PAGE)
Identification (ID)
Identification (ID)
Sequence
(Gene Sequencing)
Sequence
(Gene Sequencing)
Sequence
(Immunogen Sequence)
Sequence
(Immunogen Sequence)
Product Categories/Family for RNASEH recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 62.1kDa
Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
ribonuclease H1 isoform 1
NCBI Official Synonym Full Names
ribonuclease H1
NCBI Official Symbol
RNASEH1
NCBI Official Synonym Symbols
RNH1; H1RNA; PEOB2
NCBI Protein Information
ribonuclease H1
UniProt Protein Name
Ribonuclease H1
Protein Family
UniProt Gene Name
RNASEH1
UniProt Synonym Gene Names
RNH1; RNase H1
UniProt Entry Name
RNH1_HUMAN
NCBI Description
This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and plays a key role in DNA replication and repair. Alternate in-frame start codon initiation results in the production of alternate isoforms that are directed to the mitochondria or to the nucleus. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF). Mutations in this gene have been found in individuals with progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2. Alternative splicing results in additional coding and non-coding transcript variants. Pseudogenes of this gene have been defined on chromosomes 2 and 17. [provided by RefSeq, Jul 2017]
Research Articles on RNASEH
Similar Products
Product Notes
The RNASEH rnaseh1 (Catalog #AAA2122592) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Ribonuclease H (RNASEH) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Ribonuclease H can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the RNASEH rnaseh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease H, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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