Typical Testing Data/Standard Curve (for reference only)
Human Cyclin dependent kinase 13 (CDC2L5) ELISA Kit | CDC2L5 elisa kit
Human Cyclin dependent kinase 13 (CDC2L5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CDC2L5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CDC2L5 antibody and an CDC2L5-HRP conjugate. The assay sample and buffer are incubated together with CDC2L5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC2L5 concentration since CDC2L5 from samples and CDC2L5-HRP conjugate compete for the anti-CDC2L5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC2L5 from the sample, fewer sites are left to bind CDC2L5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC2L5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are well known for their essential roles as master switches in cell cycle control. The exact function of this protein has not yet been determined, but it may play a role in mRNA processing and may be involved in regulation of hematopoiesis. Alternatively spliced transcript variants have been described.[provided by RefSeq, Dec 2009]
Uniprot Description
CDK13: a CMGC protein kinase of the CDK family. It has been reported to interact with L-type cyclins. Overexpression of CDC2L5 constructs disturbs constitutive splicing and switches alternative splice site selection in vivo suggesting that it may regulate alternative splicing. It has unusually large N- and C-termini and is ubiquitously expressed in many tissues. Two alternatively spliced human isoforms have been described.
Protein type: Protein kinase, CMGC; EC 2.7.11.23; Cell cycle regulation; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Motility/polarity/chemotaxis; RNA splicing; CMGC group; CDK family; CRK7 subfamily; CDK/CRK7 subfamily
Chromosomal Location of Human Ortholog: 7p13
Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; nuclear cyclin-dependent protein kinase holoenzyme complex; cytoplasm; nuclear speck
Molecular Function: RNA polymerase subunit kinase activity; cyclin binding; protein binding; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity
Biological Process: alternative nuclear mRNA splicing, via spliceosome; viral reproduction; multicellular organismal development; positive regulation of cell proliferation; regulation of mitosis; hemopoiesis
Research Articles on CDC2L5
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Product Notes
The Human CDC2L5 cdk13 (Catalog #AAA7206250) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7206250 ELISA Kit recognizes Human CDC2L5. It is sometimes possible for the material contained within the vial of "Cyclin dependent kinase 13 (CDC2L5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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