Hamster anti-Mouse CD28 Monoclonal Antibody | anti-CD28 antibody

Syrian Hamster monoclonal CD28 antibody (allophycocyanin)

Cat. Num. AAA5305506

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD28; Monoclonal Antibody; Syrian Hamster monoclonal CD28 antibody (allophycocyanin); CD28 antibody (allophycocyanin); anti-CD28 antibody

• Host: Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 37.51
• Specificity: Mouse
• Form/Format: Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
• Concentration: 500 ug/ml (varies by lot)
• Sequence Length: 63

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: FC: <= 0.5 ug/test
• Assay Information: A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10? to 10? cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
• Conjugate: Allophycocyanin
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Staining of BALB/c splenocytes with CD3e antibody (FITC) and 0.25 ug of Golden Syrian Hamster IgG Isotype Control (left) or 0.25 ug of CD28 antibody (right). Total viable cells were used for analysis.)

Product Categories/Family for anti-CD28 antibody

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 192485
• NCBI GeneID: 12487
• Molecular Weight: 25,243 Da
• NCBI Official Full Name: CD28, partial
• NCBI Official Synonym Full Names: CD28 antigen
• NCBI Official Symbol: Cd28
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: Cd28

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1 C2|1 30.52 cM

Cellular Component: cell surface; external side of plasma membrane; immunological synapse; lipid raft; plasma membrane

Molecular Function: protease binding; protein homodimerization activity; protein kinase binding; SH3/SH2 adaptor activity

Biological Process: negative thymic T cell selection; positive regulation of alpha-beta T cell proliferation; positive regulation of inflammatory response to antigenic stimulus; positive regulation of interleukin-10 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of isotype switching to IgG isotypes; positive regulation of mitosis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of regulatory T cell differentiation; regulatory T cell differentiation; T cell activation; T cell costimulation; T cell receptor signaling pathway