ANGPTL4 recombinant protein

ANGPTL4 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120354

• Gene Names: ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
• Purity: >92% as determined by SDS-PAGE
• Synonyms: ANGPTL4; ANGPTL4 Protein; Human; Recombinant (hFc Tag); Human ANGPTL4 Protein (Fc Tag); ANGPTL2 Protein; ARP4 Protein; FIAF Protein; HARP Protein; HFARP Protein; NL2 Protein; PGAR Protein; pp1158 Protein; TGQTL Protein; UNQ171 Protein; angiopoietin-like 4; ANGPTL4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris-Citrate, 0.3 M NaCl, pH6.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Pro166-Ser406

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human ANGPTL4 (Q9BY76)(Pro166-Ser406) was expressed,with the fused Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for ANGPTL4 recombinant protein

Background: ANGPTL4, also known as ANGPTL2, is a protein with hypoxia-induced expression in endothelial cells. It contains 1 fibrinogen C-terminal domain and is expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. ANGPTL4 inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. It may act as a regulator of angiogenesis and modulate tumorigenesis. It inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. It may also exert a protective function on endothelial cells through an endocrine action. ANGPTL4 is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

References

Lichtenstein L, et al. (2010) Angptl4 Protects against Severe Proinflammatory Effects of Saturated Fat by Inhibiting Fatty Acid Uptake into Mesenteric Lymph Node Macrophages. Cell metabolism. 12(6): 580-92.Terada S, et al. (2011) Escaping Anoikis through ROS: ANGPTL4 controls integrin signaling through Nox1. Cancer Cell. 19(3):297-9.Zhu PC, et al. (2011) Angptl4 protein elevates the prosurvival intracellular O2(-):H2O2 ratio and confers anoikis resistance to tumors. Cancer Cell. 19(3):401-15.

NCBI and Uniprot Product Information

• NCBI GI #: 25008123
• NCBI GeneID: 51129
• UniProt Accession #: Q9BY76; Q53HQ6; Q53HU1; Q6UXN0; Q9HBV4; Q9NZU4; Q9Y5B3; A8MY84; B4E089; D6W670; F5H0I2
• Molecular Weight: 406
• NCBI Official Full Name: Angiopoietin-related protein 4
• NCBI Official Synonym Full Names: angiopoietin-like 4
• NCBI Official Symbol: ANGPTL4
• NCBI Official Synonym Symbols: NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
• NCBI Protein Information: angiopoietin-related protein 4; fasting-induced adipose factor; PPARG angiopoietin related protein; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; peroxisome proliferator-activated receptor (PPAR) gamma induced angi
• UniProt Protein Name: Angiopoietin-related protein 4
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL4
• UniProt Synonym Gene Names: ARP4; HFARP; PGAR; HFARP
• UniProt Entry Name: ANGL4_HUMAN

NCBI Description

This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq, Sep 2013]

Uniprot Description

ANGPTL4: Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.

Protein type: Secreted, signal peptide; Secreted; Apoptosis; Extracellular matrix; Inhibitor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: enzyme inhibitor activity; protein binding

Biological Process: positive regulation of angiogenesis; response to hypoxia; negative regulation of lipoprotein lipase activity; angiogenesis; cellular lipid metabolic process; cell differentiation; protein homooligomerization; negative regulation of apoptosis

Disease: Plasma Triglyceride Level Quantitative Trait Locus