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Typical Testing Data/Standard Curve (for reference only)

Human Cortistatin (CORT) ELISA Kit | CORT elisa kit

Human Cortistatin (CORT) ELISA Kit

Gene Names
CORT; CST-14; CST-17; CST-29
Reactivity
Human
Synonyms
Cortistatin (CORT); Human Cortistatin (CORT) ELISA Kit; CST14; CST17; CST29; CORT elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Cortistatin (CORT).
No significant cross-reactivity or interference between Cortistatin (CORT) and analogues was observed.
Sequence Length
105
Assay Type
Competitive Inhibition
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
222.22-18000pg/mL
Sensitivity
< 86.7pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Cortistatin (CORT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Cortistatin (CORT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CORT elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cortistatin (CORT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cortistatin (CORT) and unlabeled Cortistatin (CORT) (Standards or samples) with the pre-coated antibody specific to Cortistatin (CORT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cortistatin (CORT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cortistatin (CORT) in the sample.
Product Categories/Family for CORT elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
11,532 Da
NCBI Official Full Name
Cortistatin
NCBI Official Synonym Full Names
cortistatin
NCBI Official Symbol
CORT
NCBI Official Synonym Symbols
CST-14; CST-17; CST-29
NCBI Protein Information
cortistatin
UniProt Protein Name
Cortistatin
Protein Family
UniProt Gene Name
CORT
UniProt Entry Name
CORT_HUMAN

NCBI Description

This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]

Uniprot Description

CORT: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs. Belongs to the somatostatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: extracellular space

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding

Biological Process: synaptic transmission; G-protein signaling, adenylate cyclase inhibiting pathway

Research Articles on CORT

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Product Notes

The Human CORT cort (Catalog #AAA2022256) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2022256 ELISA Kit recognizes Human CORT. It is sometimes possible for the material contained within the vial of "Cortistatin (CORT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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