Rat CORT ELISA Kit | CORT elisa kit

Rat CORT (Corticosterone) ELISA Kit

Cat. Num. AAA2515406

• Gene Names: CORT; CST-14; CST-17; CST-29
• Reactivity: Rat
• Synonyms: CORT; Rat CORT (Corticosterone) ELISA Kit; CORT elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes Rat and Chicken CORT in samples. No significant cross-reactivity or interference between CORT and analogues was observed.
• Sequence Length: 105

• Assay Type: Competitive
• Detection Range: 0.39-25ng/mL
• Sensitivity: 0.24ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 rat samples with low, mid range and high level CORT were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 rat samples with low, mid range and high level CORT were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CORT elisa kit

Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CORT. During the reaction, CORT in the sample or standard competes with a fixed amount of CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CORT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CORT in the samples is then determined by comparing the OD of the samples to the standard curve.

Background/Introduction: Corticosterone, also known as 17-deoxycortisol, it is a steroid hormone secreted by the outer layer, or cortex, of the adrenal gland. Classed as a glucocorticoid, corticosterone helps regulate the conversion of amino acids into carbohydrates and glycogen by the liver, and helps stimulate glycogen formation in the tissues [1]. Corticosterone is similar in structure, although somewhat less potent, than the other glucocorticoids cortisol and cortisone. It is produced in response to stimulation by the pituitary substance adrenocorticotropic hormone (ACTH). In some species, but not in humans, corticosterone is the predominant glucocorticoid secreted by the adrenal. It is a precursor in the synthesis of aldosterone, another adrenal cortical steroid. The increase in corticosterone is linked to anxiety relief. This finding depends on the time at which the administration of corticosterone took place as compared to when the fear conditioning took place, corticosterone can either facilitate or interrupt conditioned fear [2].

NCBI and Uniprot Product Information

• NCBI GI #: 311893369
• NCBI GeneID: 1325
• NCBI Accession #: NP_001293.3
• NCBI GenBank Nucleotide #: NM_001302.4
• UniProt Accession #: O00230; Q5T6G0; Q6UX11
• Molecular Weight: 11,532 Da
• NCBI Official Full Name: cortistatin preproprotein
• NCBI Official Synonym Full Names: cortistatin
• NCBI Official Symbol: CORT
• NCBI Official Synonym Symbols: CST-14; CST-17; CST-29
• NCBI Protein Information: cortistatin; cortistatin-14; cortistatin-17; cortistatin-29; prepro-cortistatin; preprocortistatin
• UniProt Protein Name: Cortistatin
• Protein Family: Cortexin
• UniProt Gene Name: CORT
• UniProt Entry Name: CORT_HUMAN

NCBI Description

This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]

Uniprot Description

CORT: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs. Belongs to the somatostatin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: extracellular space

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding

Biological Process: synaptic transmission; G-protein signaling, adenylate cyclase inhibiting pathway