PRINCIPLE OF THE ASSAY This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CORT. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated CORT. A competitive inhibition reaction is launched between CORT (Standards or samples) and Biotin-conjugated CORT with the pre-coated antibody specific for CORT. The more amount of CORT in samples, the less antibody bound by Biotin-conjugated CORT. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of CORT in the sample. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]
Uniprot Description
Function: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs.
Research Articles on CORT
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Product Notes
The Rabbit CORT cort (Catalog #AAA704261) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704261 ELISA Kit recognizes Rabbit CORT. It is sometimes possible for the material contained within the vial of "Corticosterone, CORT, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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