Neutrophil Elastase/ELA2 Recombinant Protein | ELA2 recombinant protein

Recombinant Neutrophil Elastase/ELA2

Cat. Num. AAA201138

• Gene Names: Elane; NE; Ela2; F430011M15Rik
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Neutrophil Elastase/ELA2; Recombinant Neutrophil Elastase/ELA2; Elane; Ela2; F430011M15Rik; NE; Neutrophil elastase; Elastase-2; Leukocyte elastase; ELA2 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In 20mM Tris-HCl Buffer (pH 8.0) Containing 0.1M NaCl, 30% Glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 27-265aa
• Sequence: SEIVGGRPAR PHAWPFMASL QRRGGHFCGA TLIARNFVMS AAHCVNGLNF RSVQVVLGAH DLRRQERTRQ TFSVQRIFEN GFDPSQLLND IVIIQLNGSA TINANVQVAQ LPAQGQGVGD RTPCLAMGWG RLGTNRPSPS VLQELNVTVV TNMCRRRVNV CTLVPRRQAG ICFGDSGGPL VCNNLVQGID SFIRGGCGSG LYPDAFAPVA EFADWINSII RSHNDHLLTH PKDREGRTN H HHHHH
• Sequence Length: 265

• Applicable Applications for ELA2 recombinant protein: SDS-PAGE
• Species: Mouse
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ser 27
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for ELA2 recombinant protein

Neutrophil Elastase, also known as polymorphonuclear leukocyte eslastase, is a serine protease that classified as the chymotrypsin family. This protein breaks down elastin, an elastic fiber together with collagen and determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E Coli and other Gram-negative bacteria. Also, this protein is inhibited by the acute-phase protein alpha1-antitrypsin (A1AT), which binds almost irreversibly to the active site of elastase and trypsin. A1AT is normally secreted by the liver cells into the serum. Alpha-1-antitrypsin deficiency (A1AD) leads to uninhibited destruction of elastic fiber by elastase. Recombinant Mouse Neutrophil Elastase, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ELA2 recombinant protein

Immunology

References

Wiesmeier M., et al, (2016) PLoS ONE 11:e0168055. Sturrock A., et al, (1998) Cytogenet. Cell Genet. 83:104-108.

NCBI and Uniprot Product Information

• NCBI GI #: 114052444
• NCBI GeneID: 50701
• NCBI Accession #: NP_056594.2
• NCBI GenBank Nucleotide #: NP_056594.2
• UniProt Accession #: Q3UP87
• Molecular Weight: 26.8kDa (245aa); 28-40kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: neutrophil elastase
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: Elane
• NCBI Official Synonym Symbols: NE; Ela2; F430011M15Rik
• NCBI Protein Information: neutrophil elastase
• UniProt Protein Name: Neutrophil elastase
• UniProt Gene Name: Elane
• UniProt Synonym Gene Names: Ela2
• UniProt Entry Name: ELNE_MOUSE

NCBI Description

This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]

Uniprot Description

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Protease; EC 3.4.21.37; Cell cycle regulation; Motility/polarity/chemotaxis; Cell surface

Cellular Component: cell surface; cytoplasm; extracellular space; secretory granule; transcriptional repressor complex

Molecular Function: cytokine binding; endopeptidase activity; heparin binding; hydrolase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: acute inflammatory response to antigenic stimulus; defense response to bacterium; defense response to fungus; leukocyte migration; leukocyte migration during inflammatory response; negative regulation of chemokine biosynthetic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of immune response; positive regulation of interleukin-8 biosynthetic process; positive regulation of smooth muscle cell proliferation; proteolysis; response to lipopolysaccharide; response to UV; response to yeast