Human Connective Tissue Growth Factor (CTGF) ELISA Kit | CTGF elisa kit

Human Connective Tissue Growth Factor (CTGF) ELISA Kit

Cat. Num. AAA455689

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Reactivity: Human
• Synonyms: Connective Tissue Growth Factor (CTGF); Human Connective Tissue Growth Factor (CTGF) ELISA Kit; Fbln2; FIBL-2; Fibulin-2; CTGF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CTGF. No significant cross-reactivity or interference between CTGF and analogues was observed.
• Sequence Length: 349

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 46.875-3000pg/mL
• Sensitivity: 17.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTGF elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTGF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CTGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CTGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CTGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CTGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503123
• NCBI GeneID: 1490
• NCBI Accession #: NP_001892.1
• NCBI GenBank Nucleotide #: NM_001901.2
• UniProt Accession #: P29279; Q6LCY0; Q96A79; Q96QX2; Q9UDL6; E1P578
• Molecular Weight: 35,205 Da
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.