Rabbit CTGF ELISA Kit | CTGF elisa kit

Rabbit CTGF (Connective Tissue Growth Factor) ELISA Kit

Cat. Num. AAA2500432

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Reactivity: Rabbit
• Synonyms: CTGF; Rabbit CTGF (Connective Tissue Growth Factor) ELISA Kit; CTGF elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit CTGF. No significant cross-reactivity or interference between Rabbit CTGF and analogues was observed.
• Sequence Length: 349

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 46.875-3000pg/mL
• Sensitivity: Min: 28.125pg/mL; Max: 3000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit CTGF concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTGF elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTGF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CTGF. You can calculate the concentration of CTGF in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503123
• NCBI GeneID: 1490
• NCBI Accession #: NP_001892.1
• NCBI GenBank Nucleotide #: NM_001901.2
• UniProt Accession #: P29279; Q6LCY0; Q96A79; Q96QX2; Q9UDL6; E1P578
• Molecular Weight: 35,205 Da
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor; CCN family member 2; IBP-8; IGF-binding protein 8; IGFBP-8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8
• UniProt Entry Name: CTGF_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6q23.1

Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; extracellular region; cell cortex; cytosol

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding

Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JNK cascade; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of stress fiber formation; positive regulation of cell proliferation; response to wounding; tissue homeostasis; response to glucose stimulus; regulation of cell growth; angiogenesis; cell adhesion; cell differentiation; positive regulation of cell activation; cartilage condensation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; positive regulation of protein amino acid phosphorylation; regulation of chondrocyte differentiation; positive regulation of cell differentiation; lung development