Human CTGF ELISA Kit | CTGF elisa kit

CTGF (Human) OmniKine ELISA Kit

Cat. Num. AAA9501836

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Reactivity: Human
• Synonyms: CTGF; CTGF (Human) OmniKine ELISA Kit; Connective tissue growth factor; CCN family member 2; Hypertrophic chondrocyte-specific protein 24; Insulin-like growth factor-binding protein 8; CCN2; HCS24; IGFBP8; CTGF elisa kit

• Reactivity: Human
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 349

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CTGF elisa kit

Principle of the Assay: The OmniKine™ Human CTGF ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Human CTGF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human CTGF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Human CTGF, also known as Connective Tissue Growth Factor, is a 349 amino acid growth factor encoded by the CTGF gene located at locus 6q23.1 on chromosome 6. Following translation, CTGF undergoes proteolytic processing where the 26 residue signal sequence is cleaved and CTGF is allowed to fold into its mature form. CTGF is characterized as a major connective tissue mitoattractant secreted by vascular endothelial cells. These growth factors typically are known to promote proliferation and differentiation of chondrocytes, mediate heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Moreover, they tend to enhance fibroblast growth factor-induced DNA synthesis. Belonging to the CCN family, the CTGF protein contains a C-terminal cysteine knot-like domain (CTCK), an IGFBP N-terminal domain, a TSP type-1 domain, and a VWFC domain. Furthermore, this particular growth factor is mainly expressed in bone marrow and thymic cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4503123
• NCBI GeneID: 1490
• NCBI Accession #: NP_001892.1
• NCBI GenBank Nucleotide #: NM_001901.2
• UniProt Accession #: P29279; Q6LCY0; Q96A79; Q96QX2; Q9UDL6; E1P578
• Molecular Weight: 35,205 Da
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor
• UniProt Protein Name: Connective tissue growth factor
• Protein Family: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q23.2

Cellular Component: cell cortex; cis-Golgi network; cytosol; extracellular region; extracellular space; Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; proteinaceous extracellular matrix

Molecular Function: fibronectin binding; growth factor activity; heparin binding; insulin-like growth factor binding; integrin binding; protein binding; protein C-terminus binding

Biological Process: aging; angiogenesis; cartilage condensation; cell adhesion; cell differentiation; cell migration; cell-cell signaling; cell-matrix adhesion; epidermis development; fibroblast growth factor receptor signaling pathway; integrin-mediated signaling pathway; lung development; ossification; positive regulation of cell activation; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of JNK cascade; positive regulation of stress fiber formation; regulation of cell growth; regulation of chondrocyte differentiation; regulation of lipid metabolic process; response to amino acid; response to anoxia; response to estradiol; response to glucose stimulus; response to mineralocorticoid stimulus; response to peptide hormone; response to wounding; tissue homeostasis; transcription initiation from RNA polymerase II promoter