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Typical Testing Data/Standard Curve (for reference only)

Rat IL-6 ELISA Kit | IL6 elisa kit

Rat IL-6 ELISA Kit

Gene Names
Il6; ILg6; Ifnb2
Reactivity
Rat
Applications
ELISA
Synonyms
IL-6; Rat IL-6 ELISA Kit; IFNB2; Interleukin-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2; IL6 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
The Rat CNTF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat CNTF proteins within the range of 31.2 pg/ml-2000 pg/ml.
Sequence Length
211
Applicable Applications for IL6 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
10 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL6 elisa kit
Principle of the Assay: The MyBioSource's Rat CNTF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CNTF in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,357 Da
NCBI Official Full Name
Interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
Protein Family
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT

NCBI Description

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().

Research Articles on IL6

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Product Notes

The Rat IL6 il6 (Catalog #AAA8244673) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244673 ELISA Kit recognizes Rat IL6. AAA Biotech's IL-6 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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