Human Complement component C7 ELISA Kit | C7 elisa kit

Human Complement component C7 ELISA Kit

Cat. Num. AAA765771

• Reactivity: Human
• Synonyms: Complement component C7; Human Complement component C7 ELISA Kit; C7 (Complement component C7); C7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C7. No significant cross-reactivity or interference between C7 and analogues was observed.
• Sequence Length: 843

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.563-100ng/ml
• Sensitivity: 0.938ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C7 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 45580688
• NCBI GeneID: 730
• NCBI Accession #: NP_000578.2
• NCBI GenBank Nucleotide #: NM_000587.2
• Molecular Weight: 93,518 Da
• NCBI Official Full Name: complement component C7
• NCBI Official Synonym Full Names: complement component 7
• NCBI Official Symbol: C7
• NCBI Protein Information: complement component C7
• UniProt Protein Name: Complement component C7
• Protein Family: Complement component
• UniProt Gene Name: C7
• UniProt Entry Name: CO7_HUMAN

NCBI Description

C7 is a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. [provided by RefSeq, Jul 2008]

Uniprot Description

C7: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: extracellular region; membrane attack complex

Biological Process: complement activation; complement activation, alternative pathway; complement activation, classical pathway; cytolysis; innate immune response; regulation of complement activation

Disease: Complement Component 7 Deficiency