Sheep C7 ELISA Kit | C7 elisa kit

Sheep C7 (Complement Component 7) ELISA Kit

Cat. Num. AAA2510598

• Reactivity: Sheep
• Synonyms: C7; Sheep C7 (Complement Component 7) ELISA Kit; C7 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes natural and recombinant Sheep C7. No significant cross-reactivity or interference between Sheep C7 and analogues was observed.
• Sequence Length: 843

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 6.25-400pg/mL
• Sensitivity: Min: 3.75pg/mL; Max: 400pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C7 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep C7 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of C7. You can calculate the concentration of C7 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 45580688
• NCBI GeneID: 730
• NCBI Accession #: NP_000578.2
• NCBI GenBank Nucleotide #: NM_000587.2
• UniProt Accession #: P10643; Q6P3T5; Q92489
• Molecular Weight: 93,518 Da
• NCBI Official Full Name: complement component C7
• NCBI Official Synonym Full Names: complement component 7
• NCBI Official Symbol: C7
• NCBI Protein Information: complement component C7
• UniProt Protein Name: Complement component C7
• Protein Family: Complement component
• UniProt Gene Name: C7
• UniProt Entry Name: CO7_HUMAN

NCBI Description

C7 is a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. [provided by RefSeq, Jul 2008]

Uniprot Description

C7: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Biological Process: cellular sodium ion homeostasis; cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 7 Deficiency