C7 recombinant protein

C7 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121554

• Purity: >88% as determined by SDS-PAGE
• Synonyms: C7; C7 Protein; Human; Recombinant (His Tag); Human C7/Complement component 7 Protein (His Tag); Complement C7 Protein; complement component 7; C7 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >88% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln843

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human C7 (P10643)(Met1-Gln843) with a C-terminal polyhistidine tag was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for C7 recombinant protein

Background: Complement component 7 is a component of the complement system. It belongs to the complement C6/C7/C8/C9 family. It contains 1 EGF-like domain, 1 LDL-receptor class A domain, 1 MACPF domain, 2 Sushi (CCP/SCR) domains and 2 TSP type-1 domains. Complement component 7 serves as a membrane anchor. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. It is a constituent of MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.

Product Categories/Family for C7 recombinant protein

Complement Activation Pathways

References

Bossi F, et al. (2009) C7 is expressed on endothelial cells as a trap for the assembling terminal complement complex and may exert anti-inflammatory function. Blood. 113(15):3640-8.Kuijpers TW, et al. (2010) Complement factor 7 gene mutations in relation to meningococcal infection and clinical recurrence of meningococcal disease. Mol Immunol. 47(4):671-7.Thomas AD, et al. (2012) Characterization of a large genomic deletion in four Irish families with C7 deficiency. Mol Immunol. 50(1-2):57-9.

NCBI and Uniprot Product Information

• NCBI GI #: 45580688
• NCBI GeneID: 730
• NCBI Accession #: NP_000578.2
• NCBI GenBank Nucleotide #: NM_000587.2
• UniProt Accession #: P10643; Q6P3T5; Q92489
• Molecular Weight: 93,518 Da
• NCBI Official Full Name: complement component C7
• NCBI Official Synonym Full Names: complement component 7
• NCBI Official Symbol: C7
• NCBI Protein Information: complement component C7
• UniProt Protein Name: Complement component C7
• Protein Family: Complement component
• UniProt Gene Name: C7
• UniProt Entry Name: CO7_HUMAN

NCBI Description

C7 is a component of the complement system. It participates in the formation of Membrane Attack Complex (MAC). People with C7 deficiency are prone to bacterial infection. [provided by RefSeq, Jul 2008]

Uniprot Description

C7: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. Defects in C7 are a cause of complement component 7 deficiency (C7D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Biological Process: cellular sodium ion homeostasis; cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 7 Deficiency