Human Complement Component 6 (C6) ELISA Kit | C6 elisa kit

Human Complement Component 6 (C6) ELISA Kit

Cat. Num. AAA2022584

• Reactivity: Human
• Synonyms: Complement Component 6 (C6); Human Complement Component 6 (C6) ELISA Kit; C6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of C6. No significant cross-reactivity or interference between C6 and analogues was observed.
• Sequence Length: 934

• Samples: Human serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: <0.297ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C6 in human serum, plasma and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C6. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of C6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for C6 elisa kit

Immune molecule; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 38488662
• NCBI GeneID: 729
• UniProt Accession #: P13671
• Molecular Weight: 104,786 Da
• NCBI Official Full Name: complement component 6
• NCBI Official Synonym Full Names: complement component 6
• NCBI Official Symbol: C6
• NCBI Protein Information: complement component C6
• UniProt Protein Name: Complement component C6
• Protein Family: Complement component
• UniProt Gene Name: C6
• UniProt Entry Name: CO6_HUMAN

NCBI Description

This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]

Uniprot Description

C6: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C6 are the cause of complement component 6 deficiency (C6D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: membrane attack complex; extracellular region

Molecular Function: protein binding

Biological Process: positive regulation of angiogenesis; positive regulation of activation of membrane attack complex; in utero embryonic development; cytolysis; regulation of complement activation; innate immune response; complement activation, classical pathway; complement activation

Disease: Complement Component 6 Deficiency