Human Complement component C6 ELISA Kit | C6 elisa kit

Human Complement component C6 ELISA Kit

Cat. Num. AAA2884760

• Reactivity: Human
• Synonyms: Complement component C6; Human Complement component C6 ELISA Kit; C6; C6 elisa kit

• Reactivity: Human
• Sequence Length: 934

• Assay Type: Sandwich
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: 8.02 pg/mL
• Intra-Assay CV: <=4.2%
• Inter-Assay CV: <=6.4%
• Recovery: 88%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C6 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 169636415
• NCBI GeneID: 729
• NCBI Accession #: NP_000056.2
• NCBI GenBank Nucleotide #: NM_000065.3
• UniProt Accession #: P13671
• Molecular Weight: 104,786 Da
• NCBI Official Full Name: complement component C6
• NCBI Official Synonym Full Names: complement component 6
• NCBI Official Symbol: C6
• NCBI Protein Information: complement component C6
• UniProt Protein Name: Complement component C6
• Protein Family: Complement component
• UniProt Gene Name: C6
• UniProt Entry Name: CO6_HUMAN

NCBI Description

This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]

Uniprot Description

C6: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C6 are the cause of complement component 6 deficiency (C6D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: extracellular region; membrane attack complex

Molecular Function: protein binding

Biological Process: complement activation; complement activation, classical pathway; cytolysis; in utero embryonic development; innate immune response; positive regulation of activation of membrane attack complex; positive regulation of angiogenesis; regulation of complement activation

Disease: Complement Component 6 Deficiency