Human Collagen Type XVII (COL17) ELISA Kit | COL17 elisa kit

Human Collagen Type XVII (COL17) ELISA Kit

Cat. Num. AAA2020450

• Gene Names: COL17A1; BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
• Reactivity: Human
• Synonyms: Collagen Type XVII (COL17); Human Collagen Type XVII (COL17) ELISA Kit; COL17A1; BP180; BPAG2; LAD-1; LABD97; Collagen Alpha-1(XVII)chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; Linear IgA disease antigen 1; COL17 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type XVII (COL17).; No significant cross-reactivity or interference between Collagen Type XVII (COL17) and analogues was observed.
• Sequence Length: 1497

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.13ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XVII (COL17) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XVII (COL17) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL17 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XVII (COL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XVII (COL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XVII (COL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type XVII (COL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COL17 elisa kit

Signal transduction; Metabolic pathway; Developmental science; Nutrition metabolism; Dermatology

NCBI and Uniprot Product Information

• NCBI GI #: 119569999
• NCBI GeneID: 1308
• Molecular Weight: 150,447 Da
• NCBI Official Full Name: collagen, type XVII, alpha 1, isoform CRA_b
• NCBI Official Synonym Full Names: collagen, type XVII, alpha 1
• NCBI Official Symbol: COL17A1
• NCBI Official Synonym Symbols: BP180; BPA-2; BPAG2; LAD-1; BA16H23.2
• NCBI Protein Information: collagen alpha-1(XVII) chain
• UniProt Protein Name: Collagen, type XVII, alpha 1, isoform CRA_b
• UniProt Gene Name: COL17A1
• UniProt Entry Name: D3DRA2_HUMAN

NCBI Description

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]

Uniprot Description

COL17A1: May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane. Defects in COL17A1 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB). GABEB is a non- lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: collagen; hemidesmosome; endoplasmic reticulum lumen; integral to plasma membrane; extracellular region; plasma membrane; basement membrane; intercellular junction

Molecular Function: protein binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; epidermis development; extracellular matrix organization and biogenesis; hemidesmosome assembly; cell-matrix adhesion

Disease: Epidermolysis Bullosa, Junctional, Non-herlitz Type