Human Collagen Type XI Alpha 2 (COL11a2) ELISA Kit | COL11a2 elisa kit

Human Collagen Type XI Alpha 2 (COL11a2) ELISA Kit

Cat. Num. AAA2024922

• Gene Names: COL11A2; HKE5; PARP; STL3; FBCG2; DFNA13; DFNB53
• Reactivity: Human
• Synonyms: Collagen Type XI Alpha 2 (COL11a2); Human Collagen Type XI Alpha 2 (COL11a2) ELISA Kit; COL11-A2; DFNA13; DFNB53; HKE5; Collagen Alpha-2(XI)chain; COL11a2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type XI Alpha 2 (COL11a2).; No significant cross-reactivity or interference between Collagen Type XI Alpha 2 (COL11a2) and analogues was observed.
• Sequence Length: 1650

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.067ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XI Alpha 2 (COL11a2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XI Alpha 2 (COL11a2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL11a2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XI Alpha 2 (COL11a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XI Alpha 2 (COL11a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XI Alpha 2 (COL11a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type XI Alpha 2 (COL11a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COL11a2 elisa kit

Metabolic pathway; Developmental science; Nutrition metabolism; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 66347235
• NCBI GeneID: 1302
• Molecular Weight: 162,185 Da
• NCBI Official Full Name: collagen type XI alpha 2
• NCBI Official Synonym Full Names: collagen, type XI, alpha 2
• NCBI Official Symbol: COL11A2
• NCBI Official Synonym Symbols: HKE5; PARP; STL3; FBCG2; DFNA13; DFNB53
• NCBI Protein Information: collagen alpha-2(XI) chain
• UniProt Protein Name: Collagen alpha-2(XI) chain
• Protein Family: Collagen
• UniProt Gene Name: COL11A2
• UniProt Entry Name: Q4VXY6_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009]

Uniprot Description

COL11A2: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils. Defects in COL11A2 are the cause of Stickler syndrome type 3 (STL3). STL3 is an autosomal dominant non- ocular form of Stickler syndrome. Classical Stickler syndrome associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular symptoms are absent in STL3. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Defects in COL11A2 are the cause of autosomal recessive otospondylomegaepiphyseal dysplasia (OSMED). OSMED is a skeletal dysplasia accompanied by severe hearing loss. The phenotype overlaps that of autosomal dominant skeletal disorders (Stickler and Marshall syndromes) but can be distinguished by disproportionately short limbs and lack of ocular involvement. Defects in COL11A2 are the cause of Weissenbacher- Zweymueller syndrome (WZS). WZS is an autosomal dominant disorder allelic with STL3 and OSMED. WZS is also referred to as heterozygous OSMED. Defects in COL11A2 are the cause of deafness autosomal dominant type 13 (DFNA13). DFNA13 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in COL11A2 are the cause of deafness autosomal recessive type 53 (DFNB53). Defects in COL11A2 are the cause of fibrochondrogenesis type 2 (FBCG2). A severe skeletal dysplasia characterized by a flat midface, short long bones, short ribs with broad metaphyses, and vertebral bodies that show distinctive hypoplastic posterior ends and rounded anterior ends, giving the vertebral bodies a pinched appearance on lateral radiographic views. The chest is small, causing perinatal respiratory problems which usually, but not always, result in lethality. Affected individuals who survive the neonatal period have high myopia, mild to moderate hearing loss, and severe skeletal dysplasia. Belongs to the fibrillar collagen family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: collagen type XI; endoplasmic reticulum lumen; extracellular region

Molecular Function: protein binding, bridging; protein binding; metal ion binding; extracellular matrix structural constituent conferring tensile strength

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; collagen fibril organization; sensory perception of sound; cartilage development; palate development; soft palate development; skeletal development

Disease: Deafness, Autosomal Dominant 13; Weissenbacher-zweymuller Syndrome; Otospondylomegaepiphyseal Dysplasia; Deafness, Autosomal Recessive 53; Fibrochondrogenesis 2; Stickler Syndrome, Type Iii