Rabbit anti-Human, Mouse ATG9A Polyclonal Antibody | anti-ATG9A antibody

ATG9A, ID (ATG9A, APG9L1, Autophagy-related protein 9A, APG9-like 1, mATG9) (MaxLight 750)

Cat. Num. AAA6276291

• Gene Names: ATG9A; mATG9; APG9L1; MGD3208
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: ATG9A; Polyclonal Antibody; ID (ATG9A; APG9L1; Autophagy-related protein 9A; APG9-like 1; mATG9) (MaxLight 750); mATG9; anti-ATG9A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-ATG9A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ATG9A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259~289 amino acids from the Center of human APG9L1.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(APG9L1 Antibody (W267) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the APG9L1 antibody detected the APG9L1 protein (arrow).)

Western Blot (WB)

(APG9L1 Antibody (W267) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the APG9L1 antibody detected the APG9L1 protein (arrow).)

Related Product Information for anti-ATG9A antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Apg9 plays a direct role in the formation of the cytoplasm to vacuole targeting and autophagic vesicles, possibly serving as a marker for a specialized compartment essential for these vesicle-mediated alternative targeting pathways.

Product Categories/Family for anti-ATG9A antibody

Antibodies; Autophagy

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). Greenberg JT. Dev Cell. 8(6):799-801. (2005). Levine B. Cell. 120(2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 116089287
• NCBI GeneID: 79065
• NCBI Accession #: NP_076990
• NCBI GenBank Nucleotide #: NM_024085.4
• UniProt Accession #: Q7Z3C6; Q3ZAQ6; Q6P0N7; Q7Z317; Q7Z320; Q8NDK6; Q8WU65; Q9BVL5; Q9H6L1; Q9HAG7
• Molecular Weight: 60,694 Da
• NCBI Official Full Name: autophagy-related protein 9A
• NCBI Official Synonym Full Names: autophagy related 9A
• NCBI Official Symbol: ATG9A
• NCBI Official Synonym Symbols: mATG9; APG9L1; MGD3208
• NCBI Protein Information: autophagy-related protein 9A; APG9-like 1; APG9 autophagy 9-like 1; autophagy 9-like 1 protein; ATG9 autophagy related 9 homolog A
• UniProt Protein Name: Autophagy-related protein 9A
• Protein Family: Autophagy-related protein
• UniProt Gene Name: ATG9A
• UniProt Synonym Gene Names: APG9L1
• UniProt Entry Name: ATG9A_HUMAN

Uniprot Description

ATG9A: Plays a role in autophagy. Cycles between a juxta- nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and FAM48A. Interacts with FAM48A. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: endoplasmic reticulum membrane; recycling endosome; membrane; late endosome membrane; late endosome; integral to membrane; autophagic vacuole; cytoplasmic vesicle; trans-Golgi network; endosome

Molecular Function: protein binding

Biological Process: protein transport; protein localization in Golgi apparatus; mitochondrion degradation; innate immune response; autophagic vacuole formation