Human Collagen Type IX Alpha 3 (COL9a3) ELISA Kit | COL9a3 elisa kit

Human Collagen Type IX Alpha 3 (COL9a3) ELISA Kit

Cat. Num. AAA2023975

• Gene Names: COL9A3; IDD; MED; EDM3; DJ885L7.4.1
• Reactivity: Human
• Synonyms: Collagen Type IX Alpha 3 (COL9a3); Human Collagen Type IX Alpha 3 (COL9a3) ELISA Kit; COL9-A3; MED; EDM3; IDD; Collagen Alpha-3(IX)chain; COL9a3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type IX Alpha 3 (COL9a3).; No significant cross-reactivity or interference between Collagen Type IX Alpha 3 (COL9a3) and analogues was observed.
• Sequence Length: 684

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.34ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IX Alpha 3 (COL9a3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IX Alpha 3 (COL9a3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL9a3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IX Alpha 3 (COL9a3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IX Alpha 3 (COL9a3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IX Alpha 3 (COL9a3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IX Alpha 3 (COL9a3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COL9a3 elisa kit

Metabolic pathway; Developmental science; Nutrition metabolism; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 119595740
• NCBI GeneID: 1299
• UniProt Accession #: Q14050; Q13681; Q9H4G9; Q9UPE2
• Molecular Weight: 63,616 Da
• NCBI Official Full Name: collagen, type IX, alpha 3, isoform CRA_b
• NCBI Official Synonym Full Names: collagen, type IX, alpha 3
• NCBI Official Symbol: COL9A3
• NCBI Official Synonym Symbols: IDD; MED; EDM3; DJ885L7.4.1
• NCBI Protein Information: collagen alpha-3(IX) chain
• UniProt Protein Name: Collagen alpha-3(IX) chain
• Protein Family: Collagen
• UniProt Gene Name: COL9A3
• UniProt Entry Name: CO9A3_HUMAN

NCBI Description

This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. Mutations in this gene are associated with multiple epiphyseal dysplasia type 3. [provided by RefSeq, Jan 2010]

Uniprot Description

COL9A3: Structural component of hyaline cartilage and vitreous of the eye. Defects in COL9A3 are the cause of multiple epiphyseal dysplasia type 3 (EDM3); also known as multiple epiphyseal dysplasia with myopathy. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COL9A3 are a cause of susceptibility to intervertebral disk disease (IDD). A common musculo- skeletal disorder caused by degeneration of intervertebral disks of the lumbar spine. It results in low-back pain and unilateral leg pain. Susceptibility to intervertebral disk disease, is conferred by variant p.Arg103Trp (PubMed:11308397). Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum lumen; extracellular region

Molecular Function: extracellular matrix structural constituent conferring tensile strength

Biological Process: extracellular matrix disassembly; axon guidance; collagen catabolic process; extracellular matrix organization and biogenesis; male gonad development; female gonad development

Disease: Epiphyseal Dysplasia, Multiple, 3; Intervertebral Disc Disease