Human Collagen Type VIII Alpha 1 (COL8a1) ELISA Kit | COL8a1 elisa kit

Human Collagen Type VIII Alpha 1 (COL8a1) ELISA Kit

Cat. Num. AAA2020864

• Gene Names: COL8A1; C3orf7
• Reactivity: Human
• Synonyms: Collagen Type VIII Alpha 1 (COL8a1); Human Collagen Type VIII Alpha 1 (COL8a1) ELISA Kit; COL8-A1; Collagen Alpha-1(VIII)chain; Endothelial collagen; Vastatin; COL8a1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type VIII Alpha 1 (COL8a1).; No significant cross-reactivity or interference between Collagen Type VIII Alpha 1 (COL8a1) and analogues was observed.
• Sequence Length: 744

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type VIII Alpha 1 (COL8a1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type VIII Alpha 1 (COL8a1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL8a1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type VIII Alpha 1 (COL8a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type VIII Alpha 1 (COL8a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type VIII Alpha 1 (COL8a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type VIII Alpha 1 (COL8a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for COL8a1 elisa kit

Metabolic pathway; Developmental science; Nutrition metabolism; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 15488904
• NCBI GeneID: 1295
• UniProt Accession #: P27658; Q53XI6; Q96D07; D3DN42
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: Collagen, type VIII, alpha 1
• NCBI Official Synonym Full Names: collagen, type VIII, alpha 1
• NCBI Official Symbol: COL8A1
• NCBI Official Synonym Symbols: C3orf7
• NCBI Protein Information: collagen alpha-1(VIII) chain
• UniProt Protein Name: Collagen alpha-1(VIII) chain
• Protein Family: Collagen
• UniProt Gene Name: COL8A1
• UniProt Synonym Gene Names: C3orf7
• UniProt Entry Name: CO8A1_HUMAN

NCBI Description

This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]

Uniprot Description

COL8A1: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix

Chromosomal Location of Human Ortholog: 3q12.3

Cellular Component: extracellular matrix; intracellular membrane-bound organelle; endoplasmic reticulum lumen; collagen type VIII; extracellular region

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; epithelial cell proliferation; camera-type eye morphogenesis; angiogenesis; cell adhesion