Human Collagen Type IV Alpha 5 (COL4a5) ELISA Kit | COL4a5 elisa kit

Human Collagen Type IV Alpha 5 (COL4a5) ELISA Kit

Cat. Num. AAA2704222

• Gene Names: COL4A5; ATS; ASLN; CA54
• Reactivity: Human
• Synonyms: Collagen Type IV Alpha 5 (COL4a5); Human Collagen Type IV Alpha 5 (COL4a5) ELISA Kit; COL4-A5; ASLN; ATS; CA54; Collagen Alpha-5(IV)chain; Alport Syndrome; COL4a5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 5 (COL4a5).; No significant cross-reactivity or interference between Collagen Type IV Alpha 5 (COL4a5) and analogues was observed.
• Sequence Length: 20

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.12ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV Alpha 5 (COL4a5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV Alpha 5 (COL4a5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4a5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 5 (COL4a5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV Alpha 5 (COL4a5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 5 (COL4a5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV Alpha 5 (COL4a5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 587204
• NCBI GeneID: 1287
• UniProt Accession #: P29400; Q16006; Q16126; Q6LD84; Q7Z700; Q9NUB7
• Molecular Weight: 161,632 Da
• NCBI Official Full Name: Collagen type IV alpha 5, partial
• NCBI Official Synonym Full Names: collagen type IV alpha 5 chain
• NCBI Official Symbol: COL4A5
• NCBI Official Synonym Symbols: ATS; ASLN; CA54
• NCBI Protein Information: collagen alpha-5(IV) chain
• UniProt Protein Name: Collagen alpha-5(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A5

NCBI Description

This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: basal lamina; collagen type IV; endoplasmic reticulum lumen; extracellular region; neuromuscular junction

Molecular Function: extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix organization; neuromuscular junction development

Disease: Alport Syndrome, X-linked