Human Cluster Of Differentiation 4 (CD4) ELISA Kit | CD4 elisa kit

Human Cluster Of Differentiation 4 (CD4) ELISA Kit

Cat. Num. AAA450302

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Synonyms: Cluster Of Differentiation 4 (CD4); Human Cluster Of Differentiation 4 (CD4) ELISA Kit; CD4mut; p55; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CD4. No significant cross-reactivity or interference between CD4 and analogues was observed.
• Sequence Length: 458

• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.127ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD4 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD4

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.(Microbial infection) Acts as a receptor for human immunodeficiency virus-1 (PubMed:2214026, PubMed:16331979, PubMed:9641677). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).