Human C4BPalpha (C4 Binding Protein Alpha) CLIA Kit | C4BPa clia kit
Human C4BPalpha (C4 Binding Protein Alpha) CLIA Kit
Gene Names
C4bpa; C4BP
Reactivity
Human
Synonyms
C4BPalpha (C4 Binding Protein Alpha); Human C4BPalpha (C4 Binding Protein Alpha) CLIA Kit; C4BPA; Human C4BPa (C4 Binding Protein Alpha) CLIA Kit; Human C4BPa; C4BPa clia kit
Reactivity
Human
Specificity
This kit recognizes natural and recombinant C4BPalpha. No significant cross-reactivity or interference between C4BPalpha and analogues was observed.
Sequence Length
601
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for C4BPa clia kit
Intended Uses: This CLIA kit applies to the in vitro quantitative determination of C4BPalpha concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to C4BPalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4BPalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4BPalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of C4BPalpha. You can calculate the concentration of C4BPalpha in the samples by comparing the RLU value of the samples to the standard curve.
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to C4BPalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4BPalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4BPalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of C4BPalpha. You can calculate the concentration of C4BPalpha in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,266 Da
NCBI Official Full Name
C4b-binding protein alpha chain
NCBI Official Synonym Full Names
complement component 4 binding protein, alpha
NCBI Official Symbol
C4bpa
NCBI Official Synonym Symbols
C4BP
NCBI Protein Information
C4b-binding protein alpha chain
UniProt Protein Name
C4b-binding protein alpha chain
Protein Family
UniProt Gene Name
C4bpa
UniProt Synonym Gene Names
C4bp
UniProt Entry Name
C4BPA_RAT
NCBI Description
regulatory protein for the complement system of the innate immune response [RGD, Feb 2006]
Uniprot Description
Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.
