Western Blot (WB)
(HRASLS3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the HRASLS3 antibody detected HRASLS3 protein (arrow).)
Rabbit anti-Human, Mouse HRASLS3 Polyclonal Antibody | anti-HRASLS3 antibody
HRASLS3, CT (PLA2G16, HRASLS3, HREV107, Group XVI phospholipase A1/A2, Adipose-specific phospholipase A2, H-rev 107 protein homolog, HRAS-like suppressor 1, HRAS-like suppressor 3, HREV107-1, HREV107-3, Renal carcinoma antigen NY-REN-65) (AP)
Gene Names
PLA2G16; AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
HRASLS3; Polyclonal Antibody; CT (PLA2G16; HREV107; Group XVI phospholipase A1/A2; Adipose-specific phospholipase A2; H-rev 107 protein homolog; HRAS-like suppressor 1; HRAS-like suppressor 3; HREV107-1; HREV107-3; Renal carcinoma antigen NY-REN-65) (AP); PLA2G16; Renal carcinoma antigen NY-REN-65; anti-HRASLS3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-HRASLS3 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
HRASLS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human HRASLS3.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(HRASLS3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the HRASLS3 antibody detected HRASLS3 protein (arrow).)
Western Blot (WB)
(HRASLS3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the HRASLS3 antibody detected HRASLS3 protein (arrow).)
Flow Cytometry (FC/FACS)
(HRASLS3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(HRASLS3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-HRASLS3 antibody
PLA2G16 specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. It also has a weak lysophospholipase activity (By similarity). Tumor suppressor that may be involved in interferon-dependent cell death.
Product Categories/Family for anti-HRASLS3 antibody
References
Uyama, T., et al. Biochim. Biophys. Acta 1791(12):1114-1124(2009). Duncan, R.E., et al. J. Biol. Chem. 283(37):25428-25436(2008). Nazarenko, I., et al. J. Cell. Sci. 120 (PT 8), 1393-1404 (2007). Nazarenko, I., et al. Am. J. Pathol. 169(4):1427-1439(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,937 Da
NCBI Official Full Name
HRAS-like suppressor 3
NCBI Official Synonym Full Names
phospholipase A2 group XVI
NCBI Official Symbol
PLA2G16
NCBI Official Synonym Symbols
AdPLA; HRSL3; HRASLS3; HREV107; HREV107-1; HREV107-3; H-REV107-1
NCBI Protein Information
HRAS-like suppressor 3
UniProt Protein Name
HRAS-like suppressor 3
UniProt Gene Name
PLA2G16
UniProt Synonym Gene Names
HRASLS3; HREV107; HRSL3; AdPLA
UniProt Entry Name
HRSL3_HUMAN
Uniprot Description
PLA2G16: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue. N- and O- acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Belongs to the H-rev107 family.
Protein type: EC 3.1.1.32; EC 3.1.1.4; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q12.3
Cellular Component: endoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane; cytosol
Molecular Function: phospholipase A2 activity; protein binding; phospholipase A1 activity
Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; lipid catabolic process; negative regulation of cell cycle
Research Articles on HRASLS3
Similar Products
Product Notes
The HRASLS3 pla2g16 (Catalog #AAA6308331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HRASLS3, CT (PLA2G16, HRASLS3, HREV107, Group XVI phospholipase A1/A2, Adipose-specific phospholipase A2, H-rev 107 protein homolog, HRAS-like suppressor 1, HRAS-like suppressor 3, HREV107-1, HREV107-3, Renal carcinoma antigen NY-REN-65) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HRASLS3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the HRASLS3 pla2g16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HRASLS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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