Human Claudin 8 (CLDN8) ELISA Kit | CLDN8 elisa kit

Human Claudin 8 (CLDN8) ELISA Kit

Cat. Num. AAA162739

• Gene Names: CLDN8; HEL-S-79
• Reactivity: Human
• Synonyms: Claudin 8 (CLDN8); Human Claudin 8 (CLDN8) ELISA Kit; CLDN8 elisa kit

• Reactivity: Human
• Sequence Length: 225

• Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 20ng/L-3500ng/L
• Sensitivity: 9.78ng/L
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLDN8 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Claudin 7 (also known as CLDN7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CLDN7 antibody. CLDN7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CLDN7 Antibody is added and binds to CLDN7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CLDN7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CLDN7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 40788011
• NCBI GeneID: 9073
• NCBI Accession #: NP_955360.1
• NCBI GenBank Nucleotide #: NM_199328.2
• UniProt Accession #: P56748; Q53EX7; D3DSE3
• Molecular Weight: 24,845 Da
• NCBI Official Full Name: claudin-8
• NCBI Official Synonym Full Names: claudin 8
• NCBI Official Symbol: CLDN8
• NCBI Official Synonym Symbols: HEL-S-79
• NCBI Protein Information: claudin-8
• UniProt Protein Name: Claudin-8
• Protein Family: Claudin
• UniProt Gene Name: CLDN8

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This protein plays important roles in the paracellular cation barrier of the distal renal tubule, and in the paracellular barrier to prevent sodium back-leakage in distal colon. Differential expression of this gene has been observed in colorectal carcinoma and renal cell tumors, and along with claudin-7, is an immunohistochemical marker for the differential diagnosis of chromophobe renal cell carcinoma and renal oncocytoma.[provided by RefSeq, May 2010]

Uniprot Description

Tight-junction protein required for paracellular chloride transport in the kidney. Mediates recruitment of CLDN4 to tight junction in the kidney. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.