Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit | CHRNa3 elisa kit

Human Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) ELISA Kit

Cat. Num. AAA451998

• Gene Names: CHRNA3; LNCR2; PAOD2; NACHRA3
• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Alpha 3 (CHRNa3); Human Cholinergic Receptor; Alpha 3 (CHRNa3) ELISA Kit; CHR-NA3 CHRN-A3 N-AChRA3 NAChRA3 N-AChR-A3 Neuronal Acetylcholine Receptor Alpha 3; CHRNa3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHRNa3. No significant cross-reactivity or interference between CHRNa3 and analogues was observed.
• Sequence Length: 505

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.107ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHRNa3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHRNa3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNa3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CHRNa3 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHRNa3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CHRNa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHRNa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHRNa3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19923122
• NCBI GeneID: 1136
• NCBI Accession #: NP_000734.2
• NCBI GenBank Nucleotide #: NM_000743.4
• UniProt Accession #: P32297; Q15823; Q4KMN8; Q86U77; Q96RH3; Q99553; Q9BQ93; Q9BRR4
• Molecular Weight: 55,637 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-3 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 3 subunit
• NCBI Official Symbol: CHRNA3
• NCBI Official Synonym Symbols: LNCR2; PAOD2; NACHRA3
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-3
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA3
• UniProt Synonym Gene Names: NACHRA3

NCBI Description

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

Uniprot Description

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.