Cholinergic Receptor, Nicotinic, Alpha 3 Recombinant Protein | CHRNa3 recombinant protein

Recombinant Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)

Cat. Num. AAA2033304

• Gene Names: CHRNA3; LNCR2; PAOD2; NACHRA3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Cholinergic Receptor; Nicotinic; Alpha 3; Recombinant Cholinergic Receptor; Alpha 3 (CHRNa3); CHR-NA3; CHRN-A3; N-AChRA3; NAChRA3; N-AChR-A3; Neuronal Acetylcholine Receptor Alpha 3; CHRNa3 recombinant protein

• Host: E Coli
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: SEAEHRLFE RLFEDYNEII RPVANVSDPV IIHFEVSMSQ LVKVDEVNQI METNLWLKQI WNDYKLKWNP SDYGGAEFMR VPAQKIWKPD IVLYNNAVGD FQVDDKTKAL LKYTGEVTWI PPAIFKSSCK IDVTYFPFDY QNCTMKFGSW SYDKAKIDLV LIGSSMNLKD YWESGEWAIIKAPGYKHDIK YNCCEEIYPD ITYSLYIRRL
• Sequence Length: 504

• Applicable Applications for CHRNa3 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 28.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Ser32~Leu240
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 68534213
• NCBI GeneID: 1136
• Molecular Weight: 55,637 Da
• NCBI Official Full Name: Cholinergic receptor, nicotinic, alpha 3
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 3 subunit
• NCBI Official Symbol: CHRNA3
• NCBI Official Synonym Symbols: LNCR2; PAOD2; NACHRA3
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-3
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-3
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA3
• UniProt Synonym Gene Names: NACHRA3
• UniProt Entry Name: ACHA3_HUMAN

NCBI Description

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]

Uniprot Description

nAChRA3: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 3/CHRNA3 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: cell junction; cell soma; dendrite; integral to membrane; nicotinic acetylcholine-gated receptor-channel complex; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; drug binding; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity; protein heterodimerization activity

Biological Process: behavioral response to nicotine; heart development; ion transport; locomotory behavior; nervous system development; protein heterooligomerization; regulation of acetylcholine secretion; regulation of dendrite morphogenesis; regulation of excitatory postsynaptic membrane potential; regulation of membrane potential; regulation of smooth muscle contraction; response to drug; response to inorganic substance; signal transduction; synaptic transmission; synaptic transmission involved in micturition; synaptic transmission, cholinergic; transmembrane receptor protein tyrosine kinase activation (dimerization)

Disease: Smoking As A Quantitative Trait Locus 3