Rabbit anti-Human MBNL2 Polyclonal Antibody | anti-MBNL2 antibody

MBNL2, CT (MBNL2, MBLL, MBLL39, MLP1, Muscleblind-like protein 2, Muscleblind-like protein 1, Muscleblind-like protein-like, Muscleblind-like protein-like 39) (PE)

Cat. Num. AAA6319396

• Gene Names: MBNL2; MBLL; MBLL39; PRO2032
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MBNL2; Polyclonal Antibody; CT (MBNL2; MBLL; MBLL39; MLP1; Muscleblind-like protein 2; Muscleblind-like protein 1; Muscleblind-like protein-like; Muscleblind-like protein-like 39) (PE); Muscleblind-like protein-like 39; anti-MBNL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MBNL2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: MBNL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-290 amino acids from the C-terminal region of human MBNL2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MBNL2 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MBNL2 antibody detected the MBNL2 protein (arrow).)

Immunohistochemistry (IHC)

(MBNL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBNL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MBNL2 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MBNL2 antibody

This gene encodes a C3H-type zinc finger protein, which is similar to the Drosophila melanogaster muscleblind B protein. Drosophila muscleblind is a gene required for photoreceptor differentiation. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined.

Product Categories/Family for anti-MBNL2 antibody

Antibodies; Proteins

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): He, F., et al. Protein Sci. 18(1):80-91(2009). Holt, I., et al. Am. J. Pathol. 174(1):216-227(2009). Paul, S., et al. EMBO J. 25(18):4271-4283(2006). Adereth, Y., et al. Nat. Cell Biol. 7(12):1240-1247(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 21464125
• NCBI GeneID: 10150
• NCBI Accession #: NP_659002.1; NP_997187.1
• NCBI GenBank Nucleotide #: NM_144778.3
• UniProt Accession #: Q5VZF2; Q3SXY5; Q58F19; Q8NEV3; Q8TD82
• Molecular Weight: 40,518 Da
• NCBI Official Full Name: muscleblind-like protein 2 isoform 1
• NCBI Official Synonym Full Names: muscleblind-like splicing regulator 2
• NCBI Official Symbol: MBNL2
• NCBI Official Synonym Symbols: MBLL; MBLL39; PRO2032
• NCBI Protein Information: muscleblind-like protein 2; muscleblind-like 2; muscleblind-like protein 1; muscleblind-like protein-like 39
• UniProt Protein Name: Muscleblind-like protein 2
• Protein Family: Muscleblind-like protein
• UniProt Gene Name: MBNL2
• UniProt Synonym Gene Names: MBLL; MBLL39; MLP1
• UniProt Entry Name: MBNL2_HUMAN

Uniprot Description

Function: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. RNA-binding protein that binds to 5'ACACCC-3' core sequence, termed zipcode, within the 3'UTR of ITGA3. Binds to CUG triplet repeat expansion in myotonic dystrophy muscle cells by sequestering the target RNAs. Seems to regulate expression and localization of ITGA3 by transporting it from the nucleus to cytoplasm at adhesion plaques. May play a role in myotonic dystrophy pathophysiology (DM). Ref.1 Ref.8 Ref.9

Subunit structure: Interacts with ITGA3. Ref.1

Subcellular location: Nucleus. Cytoplasm. Note: Greater concentration in the nucleus. Expressed in or near large cytoplasmic adhesion plaques. Location in the cytoplasm is microtubule-dependent. In both DM1 and DM2 patients, colocalizes with nuclear foci of retained expanded-repeat transcripts. Ref.1 Ref.7

Tissue specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Ref.7

Sequence similarities: Belongs to the muscleblind family.Contains 4 C3H1-type zinc fingers.