Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Chloride intracellular channel protein 4 (CLIC4) Standard Curve.)
Human Chloride Intracellular Channel Protein 4 (CLIC4) ELISA Kit | CLIC4 elisa kit
Human Chloride Intracellular Channel Protein 4 (CLIC4) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Chloride intracellular channel protein 4 (CLIC4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Chloride intracellular channel protein 4 (CLIC4) Standard Curve.)
Background: Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells).The 253-amino acid CLIC4 protein is 97%, 67%, and 66% identical to rat H1 and human CLIC1 and CLIC2, respectively. CLIC4 has 5 potential phosphorylation sites. Northern blot analysis revealed ubiquitous expression of a 4.5-kb CLIC4 transcript, with prominent expression in heart, placenta, and skeletal muscle as well as pancreatic carcinoma cells.
NCBI and Uniprot Product Information
NCBI Description
Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]
Uniprot Description
CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: centrosome; cell surface; nuclear matrix; microvillus; cytoplasmic vesicle membrane; mitochondrion; intercellular junction; cytosol; actin cytoskeleton; microtubule cytoskeleton; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; midbody; intracellular
Molecular Function: chloride channel activity; protein binding; voltage-gated ion channel activity
Biological Process: keratinocyte differentiation; vacuolar acidification; fertilization; branching morphogenesis of a tube; apoptosis; multicellular organism growth; chloride transport; establishment and/or maintenance of apical/basal cell polarity; angiogenesis; endothelial cell morphogenesis; cell differentiation; negative regulation of cell migration; regulation of cytoskeleton organization and biogenesis
Research Articles on CLIC4
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Product Notes
The Human CLIC4 clic4 (Catalog #AAA9716081) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716081 ELISA Kit recognizes Human CLIC4. It is sometimes possible for the material contained within the vial of "Chloride Intracellular Channel Protein 4 (CLIC4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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