CLIC4 recombinant protein

Recombinant Human CLIC4 Protein (His tag)

Cat. Num. AAA2545529

• Gene Names: CLIC4; H1; huH1; p64H1; CLIC4L; MTCLIC
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: CLIC4; Recombinant Human CLIC4 Protein (His tag); CLIC4L; DKFZp566G223; FLJ38640; H1; MTCLIC; huH1; P64h1; CLIC4 recombinant protein

• Host: E Coli
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 253

• Application Notes: The recombinant human CLIC4 consisting of 263 amino acids and migrates as an 30 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CLIC4 recombinant protein

Background: Chloride intracellular channel protein 4, also known as Intracellular chloride ion channel protein p64H1 and CLIC4, is a member of the chloride channel CLIC family. It contains one GST C-terminal domain. CLIC4 is a member of a family of intracellular chloride channels. It is regulated by p53, c-Myc, and tumor necrosis factor-alpha. CLIC4 is detected in epithelial cells from colon, esophagus and kidney (at protein level). CLIC4 has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. CLIC4 could promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Expression of CLIC4 is prominent in heart, kidney, placenta and skeletal muscle. Overexpression of CLIC4 in cancer cells inhibits tumor growth. Conversely, overexpression of CLIC4 in tumor stromal cells stimulates tumor growth. Thus, CLIC4 participates in normal and pathological processes and may serve as a useful target for therapies in disturbances of homeostasis and neoplastic transformation. Loss of CLIC4 in tumor cells and gain in tumor stroma is common to many human cancers and marks malignant progression. Up-regulation of CLIC4 in tumor stroma is coincident with myofibroblast conversion, generally a poor prognostic indicator. Reactivation and restoration of CLIC4 in tumor cells or the converse in tumor stromal cells could provide a novel approach to inhibit tumor growth.

Description: A DNA sequence encoding the human CLIC4 (Q9Y696-1) (Ala 2-Lys 253) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7330335
• NCBI GeneID: 25932
• NCBI Accession #: NP_039234.1
• NCBI GenBank Nucleotide #: NM_013943.2
• UniProt Accession #: Q9Y696; Q9UFW9; Q9UQJ6
• Molecular Weight: 28,772 Da
• NCBI Official Full Name: chloride intracellular channel protein 4
• NCBI Official Synonym Full Names: chloride intracellular channel 4
• NCBI Official Symbol: CLIC4
• NCBI Official Synonym Symbols: H1; huH1; p64H1; CLIC4L; MTCLIC
• NCBI Protein Information: chloride intracellular channel protein 4
• UniProt Protein Name: Chloride intracellular channel protein 4
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC4

NCBI Description

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008]

Uniprot Description

CLIC4: Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell- surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical- basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Belongs to the chloride channel CLIC family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: actin cytoskeleton; apical part of cell; cell surface; centrosome; cytoplasm; cytosol; intercellular junction; intracellular; microtubule cytoskeleton; microvillus; midbody; mitochondrion; nuclear matrix; perinuclear region of cytoplasm; plasma membrane

Molecular Function: glutathione transferase activity; protein binding

Biological Process: angiogenesis; cell differentiation; glutathione metabolic process; keratinocyte differentiation; negative regulation of cell migration